Taxonomic integrative and phylogenetic identification of the first recorded Triatoma rubrofasciata in Zhangzhou, Fujian Province and Maoming, Guangdong Province, China
Taxonomic integrative and phylogenetic identification of the first recorded Triatoma rubrofasciata in Zhangzhou, Fujian Province and Maoming, Guangdong Province, China
复制标题
福建漳州和广东茂名首次记录的红带锥蝽的分类学综合和系统发育鉴定
DOI：
10.1186/s40249-019-0579-8
复制
发表时间：
2019-08
影响因子：
8.1
通讯作者：
Lv Zhi Yue
中科院分区：
文献类型：
--
作者：
Hu Yue;Gao Min Zhao;Huang Ping;Zhou Hong Li;Ma Yu Bin;Zhou Min Yu;Cheng Shao Yun;Xie Han Guo;Lv Zhi Yue
背景
锥蝽亚科的大多数物种仅生活在拉丁美洲。然而，有一个物种——红带锥蝽，在美洲以及非洲和亚洲的多个港口地区都有记录。越来越多的红带锥蝽已经……
BackgroundMost species of Triatominae live exclusively in Latin America. However, one species, Triatoma rubrofasciata, has been recorded in the Americas as well as in various port areas in Africa and Asia. An increasing number of T. rubrofasciata have been reported in southern China in recent years. However, the origin of this invasive insect vector in China remains unknown, therefore, accurate identification and phylogenetic analysis of the bugs are urgently needed.MethodsA total of seven triatomine insect specimens were found and collected from Maoming City, Guangdong Province, China (GDMM) and Zhangzhou City, Fujian Province, China (FJZZ), respectively. The obtained insect vector specimens were observed under a dissecting microscope for morphological classification and then the genomic DNA was extracted, and the 16S ribosomal RNA (rRNA), 28S rRNA as well as cytochrome oxidase subunit I (COI) genes of the species were amplified and sequenced. Subsequently, molecular phylogenetic analyses based on multiple alignments of the above genes were conducted in order to identify the species and determine the phylogenetic origin approximation accurately.ResultsThe triatomine insects collected from GDMM and FJZZ were identified as Triatoma rubrofasciata using morphological and genetic analyses. All of the Chinese T. rubrofasciata captured in FJZZ, GDMM and other localities in southern China, together with a Vietnamese and Brazilian strain, formed a new, cohesive clade. T. rubrofasciata in GDMM and FJZZ are likely derived from strains found in Vietnam or Brazil.ConclusionsTo the best of our knowledge, this is the first record of the invasive insect T. rubrofasciata, which is likely derived from strains native to Vietnam or Brazil, in both Maoming City, Guangdong Province and Zhangzhou City, Fujian Province of China. A comparison of the DNA sequences of the 16 s rRNA, 28 s rRNA and COI genes confirmed the specific identification of T. rubrofasciata, and its potential origin in China is based on the phylogenetic analyses undertaken in this study. More targeted interventions and improved entomological surveillance are urgently needed to control the spread of this haematophagous insect in China.
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DOI：
10.1016/j.vprsr.2017.02.002
发表时间：
2017-05
期刊：
Veterinary parasitology, regional studies and reports
影响因子：
--
作者：
U. Chaudhry;Bradley J. van Paridon;M. Lejeune;M. Shabbir;M. Rashid;K. Ashraf;S. Ashraf;J. Gilleard;N. Sargison
通讯作者：
U. Chaudhry;Bradley J. van Paridon;M. Lejeune;M. Shabbir;M. Rashid;K. Ashraf;S. Ashraf;J. Gilleard;N. Sargison
病例报道：一例罕见的白斑蛉四龄幼虫引起的泌尿蝇蛆病
影响因子：
3.8
作者：
Zhang B;Wang L;Liu J;Xu L;Song L;Wu X;Sun X;Wu Z
通讯作者：
Wu Z
影响因子：
8.1
作者：
Liu Q;Guo YH;Zhang Y;Zhou ZB;Zhang LL;Zhu D;Zhou XN
通讯作者：
Zhou XN
影响因子：
2.7
作者：
Dujardin, Jean-Pierre;Khoa Pham Thi;Schofield, Chris J.
通讯作者：
Schofield, Chris J.
影响因子：
4.1
作者：
Hypsa, V;Tietz, DF;Jurberg, J
通讯作者：
Jurberg, J