Staphylococcus epidermidis is involved in a mechanism for female reproduction in mice
Staphylococcus epidermidis is involved in a mechanism for female reproduction in mice
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表皮葡萄球菌参与小鼠雌性生殖机制
DOI：
10.1016/j.reth.2014.12.003
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发表时间：
2015
影响因子：
4.3
通讯作者：
A Umezawa.
中科院分区：
文献类型：
--
作者：
C Ono;M Yoshida;N Kawano;K Miyado;A Umezawa.
器官的外表面和内表面（例如，皮肤、口腔、消化道和肠）被细菌覆盖，这些细菌通常导致宿主动物的生理事件。尽管外部开放的器官，哺乳动物女性生殖道中的细菌的存在是不确定的。在这里，我们使用野生型小鼠品系C57 BL/6 N和ICR来评估这个问题。我们首先证明了在有生育经验的C57 BL/6 N小鼠（“经产”）中从输卵管液中形成细菌菌落，但在没有生育经验的小鼠（“非经产”）中没有。16 S核糖体RNA（rRNA）序列分析表明，经产小鼠输卵管液中不存在表皮葡萄球菌，免疫组化结果证实了这一点。此外，腹腔注射抗生素，青霉素G和链霉素的细菌种群灭绝，扰乱了ICR小鼠胚胎的规则植入模式。我们的研究结果表明，共生表皮葡萄球菌在小鼠着床后胚胎和子宫之间的相互作用中发挥作用。
Both external and internal surfaces of organs (e.g., skin, mouth, gut, and intestine) are covered with bacteria, which often contribute to physiological events in host animals. Despite externally opened organs, the presence of bacteria in the mammalian female reproductive tract is uncertain. Here we assessed this problem using wild-type strains of mice, C57BL/6N and ICR. We first demonstrated that bacterial colonies were formed from the oviductal fluid in the C57BL/6N mice with birth experience (“parous”), but not in the mice without birth experience (“non-parous”). Sequence analysis of 16S ribosomal RNA (rRNA) revealed thatStaphylococcus epidermidisexisted in the oviductal fluid of the parous mice, confirmed by immunohistochemical analysis. Furthermore, extinction of bacterial population with intraperitoneal injection of antibiotics, penicillin G and streptomycin, disturbed the regularly implanted pattern of embryos in ICR mice. Our results indicate that symbioticS. epidermidisplays a role in interaction between embryo and uterus upon implantation in mice.