Rapid determination of oyster lifespans and growth rates using LA-ICP-MS line scans of shell Mg/Ca ratios
Rapid determination of oyster lifespans and growth rates using LA-ICP-MS line scans of shell Mg/Ca ratios
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DOI：
10.1016/j.palaeo.2017.06.013
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发表时间：
2017-11-01
影响因子：
3
通讯作者：
Dietl, Gregory P.
中科院分区：
文献类型：
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作者：
Durham, Stephen R.;Gillikin, David P.;Dietl, Gregory P.
生活史特征的回顾性估计（例如，软体动物的生长率，寿命，物候等资料，对古生物学，考古学，渔业科学等多个领域都有重要价值。对于牡蛎等缺乏可靠的年度生长形态指标的物种，获得这些数据的最佳选择是，生长线）是使用耗时且昂贵的稳定同位素分析。然而，激光烧蚀分析镁/钙比稳定同位素分析更快，更便宜，虽然有几项研究表明，镁/钙比在双壳类壳不反映水温，往往有一个弱的相关性，可能允许年度周期检测。在这里，我们探讨的实用程序的线扫描分析的Mg/Ca比激光烧蚀电感耦合等离子体质谱法（LA-ICP-MS）作为一个更快速，更便宜的方法获得个体发育年龄估计的软体动物壳比更传统的氧稳定同位素分析。我们测试了这种方法，测量镁/钙比从21个化石和现代标本的两种牡蛎，Crassostrea virginica和Magallana gigas（以前的Crassostrea gigas），收集了广泛的地理区域沿着美国海岸。我们比较了镁/钙的生长曲线与已知的寿命或从8180个配置文件估计的生长特性。这些分析表明，激光烧蚀分析的Mg/Ca剖面可靠地再现了更广泛使用的8180剖面的年度特征。总共，97%（n = 102）的所有季节性波峰和波谷，包括那些从8180配置文件和预期的模式，在已知年龄的壳，可检测到的镁/钙配置文件。我们的结论是，激光烧蚀分析镁/钙比是一种快速和具有成本效益的替代稳定同位素分析的生长特性的牡蛎和潜在的其他类群缺乏可靠的年度形态特征的贝壳的回顾性估计。(C)2017爱思唯尔B. V.保留所有权利。
Retrospective estimates of life-history traits (e.g., growth rate, lifespan, phenology) of mollusks are valuable data for a number of fields, including paleontology, archaeology, and fisheries science. The best option for obtaining these data for species such as oysters that lack reliable morphological indicators of annual accretionary growth (e.g., growth lines) is to use time consuming and expensive stable isotope analyses. However, laser ablation analyses of Mg/Ca are faster and less expensive than stable isotope analyses, and although several studies have shown Mg/Ca ratios in bivalve shells do not reflect water temperature, there is often a weak correlation that may allow annual cycles to be detected. Here, we explore the utility of line scan analyses of Mg/Ca ratios using laser ablation inductively coupled plasma-mass spectrometry (LA-ICP-MS) as a more rapid and less expensive method for obtaining ontogenetic age estimates of mollusk shells than more traditional oxygen stable isotope analyses. We tested this method by measuring Mg/Ca ratios from 21 fossil and modern specimens of two oyster species, Crassostrea virginica and Magallana gigas (formerly Crassostrea gigas), collected across a wide geographic area along the coast of the United States. We compared Mg/Ca growth profiles with either known lifespans or with growth characteristics estimated from 8180 profiles. These analyses showed that Mg/Ca profiles from laser ablation analyses reliably reproduced the annual features of the more widely used 8180 profiles. In total, 97% (n = 102) of all seasonal peaks and troughs, including both those from the 8180 profiles and the expected patterns in the shells of known age, were detectable in the Mg/Ca profiles. We conclude that laser ablation analysis of Mg/Ca ratios is a rapid and cost effective alternative to stable isotope analysis for retrospective estimation of the growth characteristics of oysters and potentially other taxa with shells lacking reliable annual morphological features. (C) 2017 Elsevier B.V. All rights reserved.