Effect of enamel matrix protein derivative on healing of surgical supra-infrabony periodontal defects in the rat molar: A histomorphometric study
Effect of enamel matrix protein derivative on healing of surgical supra-infrabony periodontal defects in the rat molar: A histomorphometric study
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DOI：
10.1902/jop.2006.050317
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发表时间：
2006-06-01
影响因子：
4.3
通讯作者：
Weinreb, Miron
中科院分区：
文献类型：
--
作者：
Nemcovsky, Carlos E.;Zahavi, Shira;Weinreb, Miron
背景：牙釉质基质蛋白衍生物（EMD）在人类和动物研究中已被证明可促进牙周再生。目前的组织形态计量学研究评估愈合的联合上，下骨牙周缺损与EMD。方法：本研究包括两组10只Wistar大鼠，7至8个月大。在上颌第一磨牙近中牙根的近中面创建骨缺损。将根面刨平，涂24%EDTA凝胶2分钟，然后用水冲洗。实验组应用EMD，对照组仅应用藻酸丙二醇酯。手术后12周处死动物，取出组织块切片，脱矿物质并包埋在石蜡中。对于组织形态计量学分析，从缺损的中心区域选择三个切片。牙根、手术缺损、上皮附着、龈沟、嵴上结缔组织、关节强直、新牙骨质和新骨的长度和面积进行了测量。其余参数计算为缺陷的百分比。实验组牙龈退缩较小（P = 0.05），龈沟较深（P = 0.05），结合上皮较短（P = 0.01），仅有实验组有新生牙骨质形成（P = 0.02）。对照组的关节强直是对照组的6倍，但无统计学显著性。结论：釉基质蛋白衍生物通过减少牙龈退缩和根面沿着上皮，促进新牙骨质的形成，促进牙周组织愈合。
Background: Enamel matrix protein derivative (EMD) has proven to enhance periodontal regeneration in human and animal studies. The present histomorphometric study evaluated healing of combined supra-infrabony periodontal defects with EMD.Methods: The study comprised two groups of 10 Wistar rats each, 7 to 8 months old. Bony defects were created on the mesial aspect of the mesial root of the first maxillary molar. The root surface was planed and 24% EDTA gel applied for 2 minutes and then rinsed with water. In the study group, EMD was applied, and in the control group, only propylene glycol alginate was applied. Animals were sacrificed 12 weeks after surgery, and block sections were removed, demineralized, and embedded in paraffin. For histomorphometric analysis, three sections from the central area of the defect were selected. Root, surgical defect, epithelial attachment, sulcus, supracrestal connective tissue, ankylosis, and the length and area of new cementum and new bone were measured.Results: No statistically significant differences between the two groups were found for root and defect measures. The remaining parameters were calculated as a percentage of the defect. In the study group, smaller gingival recession (P = 0.05), deeper gingival sulcus (P = 0.05), and shorter junctional epithelium (P = 0.01) were found. New cementum was observed in the study group only (P = 0.02). Ankylosis was six times larger in the control group but not statistically significant. New bone formation was similar in both groups.Conclusion: Enamel matrix protein derivative enhanced periodontal healing in this model by reducing gingival recession and junctional epithelium along the root surface and enhancing the formation of new cementum.