Effect of phospholipid on the redox potential and enzymic properties of cytochromes b.
Effect of phospholipid on the redox potential and enzymic properties of cytochromes b.
复制标题
磷脂对细胞色素氧化还原电位和酶性质的影响b．
DOI：
10.1016/0003-9861(79)90424-7
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发表时间：
1979
影响因子：
3.9
通讯作者：
T. King
中科院分区：
文献类型：
--
作者：
C. Yu;L. Yu;T. King
The effects of phospholipid on the redox behavior ofbcytochromes in succinate-cytochromecreductase, the cytochromeb-c1complex, and an isolated cytochromebpreparation were investigated by the oxidative and reductive titrations. ThreeEmvalues of cytochromebwere observed in the phospholipid-sufftcient and -depleted succinate-cytochromecreductase. Their midpoint potentials at pH 7.4 are 75, 75, and −100 mV for the sufficient and 10, −30, and −160 mV for the depleted reductase. The molar distribution of thebcytochromes of theseEmvalues correspond to 30, 30, and 40%, respectively. TheEmvalues of the isolated cytochromebpreparations were not affected by addition of phospholipids. The isolatedbpreparation contained two components of equal concentration withEmvalues of −85 and −200 mV. No direct correlation between enzymic activity and the amount of high potentialbcytochromes present in the systems was demonstrated. Very little difference was observed in redox behavior ofbcytochromes between the aged inactive preparations of phospholipid-depleted reductase and that of freshly prepared reconstitutively active enzyme.
