PERTURBATION OF EPIDERMAL LANGERHANS CELLS IN BASAL-CELL CARCINOMAS
PERTURBATION OF EPIDERMAL LANGERHANS CELLS IN BASAL-CELL CARCINOMAS
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DOI：
10.1097/00000372-198712000-00001
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发表时间：
1987-12-01
影响因子：
1.1
通讯作者：
KRIPKE, ML
中科院分区：
文献类型：
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作者：
AZIZI, E;BUCANA, C;KRIPKE, ML
表皮朗格汉斯细胞 (LC) 在多种局部免疫反应中发挥着关键作用。使用 ATP 酶染色制剂，我们评估了 12 名基底细胞癌 (BCC) 患者皮肤中这些细胞的密度和形态特征。在三名患者中，我们比较了肿瘤中的 LC 密度与病灶周围皮肤中的 LC 密度。在这些情况下，两个位点的LC/mm 2 数没有差异。所有样本中 ATP 酶阳性、树突状 LC 的扰动都很明显。这些扰动包括通常均匀的液晶网络的不同程度的破坏和液晶形态的改变。许多 LC 具有圆形、变形的细胞体、缩短或完全缺失的树突，以及 ATP 酶染色强度的变化。通过使用图像分析仪，根据液晶的面积和周长，获得了圆度偏差的测量值——“树状指数”(DI)。在 BCC 表面的 LC 中观察到的 DI 明显低于病变周围皮肤中存在的 LC，这意味着主要是树突较少、更圆润的 LC。这一发现提出了基底细胞癌可能发生在这些免疫细胞的形态或活性发生改变的区域的可能性。或者，扰动可以反映肿瘤细胞对液晶形态的影响。
Epidermal Langerhans cells (LC) play a pivotal role in a variety of local immune responses. Using ATPase-stained preparations, we evaluated the density and morphologic features of these cells in the skin of 12 patients with basal cell carcinoma (BCC). In three patients, we compared the density of LC in the tumor to that in the perilesional skin. In these cases, there was no difference in the numbers of LC/mm 2 in the two sites. Perturbations of ATPase-positive, dendritic LC were evident in all specimens. These perturbations included various degrees of disruption of the usually uniform LC network and alterations in the morphology of LC. Many LC had rounded, deformed cell bodies, dendrites that were shortened or completely absent, and variations in the intensity of ATPase staining. With the use of an image analyzer, a measure of the deviation from circularity—a “dendricity index”(DI)—was obtained, based on the area and perimeter of the LC. A significantly lower DI, implying a predominance of less dendritic, more rounded LC, was observed in LC at the BCC surface than in LC present in perilesional skin. This finding raises the possibility that BCC may arise in areas where the morphology, or activity, of these immune cells has been altered; alternatively, the perturbations could reflect an effect of the tumor cells on LC morphology.