Very similar strains of Halococcus salifodinae are found in geographically separated Permo-Triassic salt deposits
Very similar strains of Halococcus salifodinae are found in geographically separated Permo-Triassic salt deposits
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DOI：
10.1099/00221287-145-12-3565
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发表时间：
1999-12-01
期刊：
影响因子：
2.8
通讯作者：
Wanner, G
中科院分区：
文献类型：
--
作者：
Stan-Lotter, H;McGenity, TJ;Wanner, G
作者之前已经从大约2.5亿年前的奥地利岩盐沉积中分离出了一种新的极端嗜盐古菌，盐盐球菌Blp。在这项研究中，他们将菌株Blp与另外两种从地理上遥远的相似年龄的盐沉积中独立分离出来的盐球菌进行了比较，并与菌株Blp在同一地点的两个最近分离株（N1和H2）进行了比较，菌株BG2/2来自德国的盐矿，菌株Br3来自英国的岩盐沉积；两人都像Hc。细胞形态和群体形态。菌株Blp、BG2/2和Br3具有相同的16S rRNA序列，与其他盐球菌不同的全细胞蛋白图谱非常相似，G+C含量相似，其5S rRNA基因的108个碱基插入序列相同，其他相似之处包括极性脂质的组成和相对丰度、抗生素敏感性、酶活性和傅里叶变换红外光谱。菌株N1和H2表现出与菌株Blp、Br3和BG2/2相似的形态、全细胞蛋白模式和生化特征，其部分16S rRNA序列（分别为682和641个碱基）与菌株Blp、Br3和BG2/2难以区分，因此菌株N1和H2可以认为是Hc的再分离株。结果表明，可活的嗜盐古生菌属于同一种，分布在地质年代相似的广泛分离的蒸发岩位置，并支持这些从地下盐沉积中分离出来的嗜盐古生菌可能是居住在古代高盐海洋的种群的残余的观点。
The authors have previously isolated a novel extremely halophilic archaeon, Halococcus salifodinae Blp, from Austrian rock salt deposited about 250 million years ago, In this study they compared strain Blp with two other halococci isolated independently from geographically distant salt deposits of similar age, and with two recent isolates (N1 and H2) from the same site as strain Blp, Strain BG2/2 was from a salt mine in Germany and strain Br3 from a halite deposit in England; both resembled Hc. salifodinae Blp in cellular and colonial morphology. Strains Blp, BG2/2 and Br3 had identical 16S rRNA sequences, very similar whole-cell protein patterns, which were different from those of other halococci, similar G+C contents and identical sequences in a 108-base insertion in their 5S rRNA gene, Other similarities included composition and relative abundances of polar lipids, antibiotic susceptibility, enzymic activities and Fourier-transform infrared spectra. Strains N1 and H2 showed similar morphology, whole-cell protein patterns and biochemical characteristics as strains Blp, Br3 and BG2/2, Their partial 16S rRNA sequences (682 and 641 bases, respectively) were indistinguishable from those of strains Blp, Br3 and BG2/2, Therefore strains N1 and H2 can be considered as reisolates of Hc. salifodinae which were obtained 8 years after the first samples were taken from that mine, The results presented suggest that viable halophilic archaea, which belong to the same species, occur in widely separated evaporite locations of similar geological age, and support the notion that these halophilic isolates from subterranean salt deposits may be the remnants of populations which inhabited ancient hypersaline seas.