Hindlimb spasticity after unilateral motor cortex lesion in rats is reduced by contralateral nerve root transfer.
Hindlimb spasticity after unilateral motor cortex lesion in rats is reduced by contralateral nerve root transfer.
复制标题
对侧神经根转移可减轻大鼠单侧运动皮层损伤后的后肢痉挛
DOI：
10.1042/bsr20160412
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发表时间：
2016-12
影响因子：
4
通讯作者：
Hou C
中科院分区：
文献类型：
--
作者：
Zong H;Ma F;Zhang L;Lu H;Gong J;Cai M;Lin H;Zhu Y;Hou C
健侧神经根移位至痉挛侧可减轻一侧运动皮层损伤引起的对侧后肢痉挛。下肢痉挛是后天性脑损伤患者常见的后遗症。最佳治疗方法仍有争议。本研究旨在探讨健侧神经根移位减轻大鼠脑卒中后患肢肌痉挛的可行性和有效性。在本研究中，我们首次建立了一种新的大鼠后肢痉挛性偏瘫模型，并建立了一种新的手术方法来减少运动皮层损伤引起的大鼠后肢痉挛性偏瘫。将36只大鼠随机分为3组。A组：假手术组。B组，损毁一侧后肢运动皮质。C组：一侧后肢皮质毁损，健侧L4前根移位至患侧L5前根。采用足印分析、Hoffmann反射（H反射）、霍乱毒素B亚单位（CTB）逆行追踪腓肠肌（GM）运动神经元和CTB标记的运动神经元上囊泡谷氨酸转运体1（VGLUT 1）的免疫荧光染色来评估患肢的痉挛状态。术后16周，C组趾展度和步长较B组明显恢复（P<0.001）。C组腓肠肌和跖肌H波最大振幅（Hmax）/M波最大振幅（Mmax）比值明显降低（P<0.01）。C组运动神经元VGLUT 1阳性终末数明显减少（P<0.001）。我们首次证明，对侧L4腹根转移到L5腹根的患侧是有效的，在缓解单侧运动皮层损伤引起的大鼠后肢痉挛。我们的数据表明，这可能是一种替代治疗脑损伤后单侧下肢痉挛。因此，健侧神经移位可能是改善痉挛性偏瘫患者下肢功能的一种潜在治疗方法。
Transfer of nerve root from normal side to the spastic side could reduce unilateral motor cortex lesion-induced contralateral hindlimb spasticity in rats. Lower extremity spasticity is a common sequela among patients with acquired brain injury. The optimum treatment remains controversial. The aim of our study was to test the feasibility and effectiveness of contralateral nerve root transfer in reducing post stroke spasticity of the affected hindlimb muscles in rats. In our study, we for the first time created a novel animal hindlimb spastic hemiplegia model in rats with photothrombotic lesion of unilateral motor cortex and we established a novel surgical procedure in reducing motor cortex lesion-induced hindlimb spastic hemiplegia in rats. Thirty six rats were randomized into three groups. In group A, rats received sham operation. In group B, rats underwent unilateral hindlimb motor cortex lesion. In group C, rats underwent unilateral hindlimb cortex lesion followed by contralateral L4 ventral root transfer to L5 ventral root of the affected side. Footprint analysis, Hoffmann reflex (H-reflex), cholera toxin subunit B (CTB) retrograde tracing of gastrocnemius muscle (GM) motoneurons and immunofluorescent staining of vesicle glutamate transporter 1 (VGLUT1) on CTB-labelled motoneurons were used to assess spasticity of the affected hindlimb. Sixteen weeks postoperatively, toe spread and stride length recovered significantly in group C compared with group B (P<0.001). Hmax (H-wave maximum amplitude)/Mmax (M-wave maximum amplitude) ratio of gastrocnemius and plantaris muscles (PMs) significantly reduced in group C (P<0.01). Average VGLUT1 positive boutons per CTB-labelled motoneurons significantly reduced in group C (P<0.001). We demonstrated for the first time that contralateral L4 ventral root transfer to L5 ventral root of the affected side was effective in relieving unilateral motor cortex lesion-induced hindlimb spasticity in rats. Our data indicated that this could be an alternative treatment for unilateral lower extremity spasticity after brain injury. Therefore, contralateral neurotization may exert a potential therapeutic candidate to improve the function of lower extremity in patients with spastic hemiplegia.