Pore-forming toxin-like protein complex expressed by frog promotes tissue repair
Pore-forming toxin-like protein complex expressed by frog promotes tissue repair
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青蛙表达的成孔毒素样蛋白复合物促进组织修复
DOI：
10.1096/fj.201800087r
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发表时间：
2019-01-01
期刊：
影响因子：
4.8
通讯作者：
Zhang, Yun
中科院分区：
文献类型：
--
作者：
Gao, Zhen-Hua;Deng, Cheng-Jie;Zhang, Yun
组织修复是一个高度动态的过程，急性炎症的立即发作被认为是修复的必要条件。孔形成蛋白在病原体入侵和宿主免疫中都很重要。然而，它们在伤口愈合和组织修复中的作用尚不清楚。-结晶蛋白融合溶气蛋白样蛋白（-亚单位）和三叶因子（-亚单位）复合体（-CAT）是一种细菌成孔毒素溶气蛋白样蛋白和三叶因子的复合体，在大鲵中发现。本研究通过建立小鼠皮肤创面模型，探讨-CAT对皮肤创面愈合的影响。-CAT通过改善再上皮化加速全层创面愈合。这种复合物缓解了真皮水肿，促进无疤痕愈合。-CAT治疗导致IL-1的快速释放，在愈合早期引发急性炎症反应。同时，-CAT处理后，创面周围TGF-1、VEGF、bFGF的表达水平和M2巨噬细胞的募集均显著增加。-CAT通过改善伤口部位的中性粒细胞募集来保护皮肤伤口免受耐甲氧西林金黄色葡萄球菌的侵袭。总之，我们的研究结果表明-CAT可以通过引发急性炎症反应来促进组织修复和保护皮肤伤口免受抗生素耐药细菌感染。这是首次发现广泛存在于动植物中的类气溶素成孔蛋白可能在伤口愈合和组织修复中起作用。高,Z.-H。邓，c.j.。，谢，y - y。郭晓龙；Wang, q - q。刘丽珍。李，w - h。李，s, a。青蛙表达的成孔毒素样蛋白复合物促进组织修复。
Tissue repair is a highly dynamic process, and the immediate onset of acute inflammation has been considered necessary for repair. Pore-forming proteins are important, both in pathogen invasion and host immunity. However, their roles in wound healing and tissue repair are unclear. -crystallin fused aerolysin-like protein (-subunit) and trefoil factor (-subunit) complex (-CAT) is a complex of a bacterial pore-forming toxin aerolysin-like protein and trefoil factor identified in the frog Bombina maxima. In this study, we established mouse cutaneous wound models to explore the effects of -CAT on skin wound healing. -CAT accelerated the healing of full-thickness wounds by improving re-epithelialization. This complex relieved dermal edema and promoted scarless healing. -CAT treatment resulted in a rapid release of IL-1, which initiated an acute inflammation response in the early stage of healing. Meanwhile, the expression levels of TGF-1, VEGF, and bFGF and the recruitment of M2 macrophages around the wound significantly increased after -CAT treatment. -CAT protected skin wounds against methicillin-resistant Staphylococcus aureus by improving neutrophil recruitment at the site of the wound. Overall, our results suggest that -CAT can promote tissue repair and protect skin wounds against antibiotic-resistant bacterial infection by triggering the acute inflammatory response. This is the first example that aerolysin-like pore-forming proteins widely existing in plants and animals may act in wound healing and tissue repair.Gao, Z.-H., Deng, C.-J., Xie, Y.-Y., Guo, X.-L., Wang, Q.-Q., Liu, L.-Z., Lee, W.-H., Li, S.-A., Zhang, Y. Pore-forming toxin-like protein complex expressed by frog promotes tissue repair.