2 NEUROTROPIC VIRUSES, HERPES-SIMPLEX VIRUS TYPE-1 AND MOUSE HEPATITIS-VIRUS, SPREAD ALONG DIFFERENT NEURAL PATHWAYS FROM THE MAIN OLFACTORY-BULB
2 NEUROTROPIC VIRUSES, HERPES-SIMPLEX VIRUS TYPE-1 AND MOUSE HEPATITIS-VIRUS, SPREAD ALONG DIFFERENT NEURAL PATHWAYS FROM THE MAIN OLFACTORY-BULB
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DOI：
10.1016/0306-4522(93)90045-h
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发表时间：
1993-12-01
期刊：
影响因子：
3.3
通讯作者：
PERLMAN, S
中科院分区：
文献类型：
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作者：
BARNETT, EM;CASSELL, MD;PERLMAN, S
几种嗜神经性病毒在外周接种后进入大脑，并沿着已知连接到最初进入部位的路径跨神经传播。在这项研究中，两种病毒，单纯疱疹病毒1型和小鼠肝炎病毒株JHM，在接种到小鼠的鼻腔或主要嗅球后，用原位杂交的方法比较了它们所利用的途径。结果表明，这两种病毒的传播都是为了感染一组独特的、仅部分重叠的主嗅球连接。已知的初级和次级主嗅球连接的感染模式在数量和质量上都有差异。用酪氨酸羟化酶免疫组织化学结合原位杂交的方法，证实只有单纯疱疹病毒感染蓝斑去甲肾上腺素能神经元。相反，这两种病毒都感染了腹侧被盖区的多巴胺能神经元，尽管小鼠肝炎病毒在A10组以及感染A8和A9组产生了更广泛的感染。结果表明，特定神经递质系统对病毒的不同摄取有助于病毒传播模式，尽管其他因素，如接触受感染细胞上特定突触的不同，以及两种病毒细胞受体分布的不同，也可能是重要的。数据显示，不同病毒的神经追踪可以从接种部位定义独特的神经路径。数据还表明，两种病毒可以通过嗅觉系统进入大脑，并定位于不同的结构，这表明涉及大脑不同区域的神经系统疾病可能是由不同的病毒引起的，即使进入发生在共同的部位。
Several neurotropic viruses enter the brain after peripheral inoculation and spread transneuronally along pathways known to be connected to the initial site of entry. In this study, the pathways utilized by two such viruses, herpes simplex virus type 1 and mouse hepatitis virus strain JHM, were compared using in situ hybridization following inoculation into either the nasal cavity or the main olfactory bulb of the mouse. The results indicate that both viruses spread to infect a unique and only partially overlapping set of connections of the main olfactory bulb. Both quantitative and qualitative differences were observed in the patterns of infection of known primary and secondary main olfactory bulb connections. Using immunohistochemistry for tyrosine hydroxylase combined with in situ hybridization, it was shown that only herpes simplex virus infected noradrenergic neurons in the locus coeruleus. In contrast, both viruses infected dopaminergic neurons in the ventral tegmental area, although mouse hepatitis virus produced a more widespread infection in the A10 group, as well as infecting A8 and A9.The results suggest that differential virus uptake in specific neurotransmitter systems contributes to the pattern of viral spread, although other factors, such as differences in access to particular synapses on infected cells and differences in the distribution of the cellular receptor for the two viruses, are also likely to be important. The data show that neural tracing with different viruses may define unique neural pathways from a site of inoculation. The data also demonstrate that two viruses can enter the brain via the olfactory system and localize to different structures, suggesting that neurological diseases involving disparate regions of the brain could be caused by different viruses, even if entry occurred at a common site.