ANIMAL-MODEL FOR ASSESSMENT OF SKIN IRRITANCY
ANIMAL-MODEL FOR ASSESSMENT OF SKIN IRRITANCY
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DOI：
10.1111/j.1600-0536.1986.tb01315.x
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发表时间：
1986-09-01
影响因子：
5.5
通讯作者：
GROTH, O
中科院分区：
文献类型：
--
作者：
ANDERSON, C;SUNDBERG, K;GROTH, O
通过肉眼和显微镜对豚鼠重复开放应用低浓度十二烷基硫酸钠（SLS）的皮肤刺激反应进行了研究。在每天3次施用持续3天后，2%SLS水溶液给出了肉眼评估，表皮厚度和总真皮炎性细胞反应的增加，其大于1%SLS溶液。真皮炎症细胞反应本质上主要是单核细胞（淋巴细胞）。以SLS反应为对照，研究了各种有机溶剂，并对SLS反应和内部进行了排序。三氯乙烯是最具刺激性的溶剂，高达2%SLS。测试的其他氯化碳氢化合物和芳香烃引起刺激性反应。醇和丙酮没有反应。白色酒精和三氯乙烯一样刺激。稀释剂的刺激性较小，约为1%SLS对照反应的水平。4天实验设计是筛选潜在刺激性的方便和合适的动物模型，并提供了刺激性接触反应发病机制的相关信息。
Irritant skin reactions from repeated open applications of low concentrations of sodium lauryl sulphate (SLS) have been studied macroscopically and microscopically in guinea pigs. After 3 applications daily for 3 days, 2% SLS aqueous solutions gave a naked eye assessment, increase in epidermal thickness and total dermal inflammatory cell response, which was greater than for a 1% SLS solution. The dermal inflammatory cell response was mainly mononuclear (lymphocytic) in nature. With the SLS reactions as control, various organic solvents were studied and ranked against the SLS reactions and internally. Trichlorethylene was the most irritant solvent, ranking as high as 2% SLS. Other chlorinated hydrocarbons and aromatic hydrocarbons tested caused irritant reactions. The alcohols and acetone gave no reaction. White spirit was as irritant as trichlorethylene. Thinners were less irritant, around the level of the 1% SLS control reaction. The 4-day experimental design is a convenient and suitable animal model for screening irritant potential, and provides information relevant to the pathogenesis of irritant contact reactions.