Hsa_circ_0001649: A circular RNA and potential novel biomarker for colorectal cancer
Hsa_circ_0001649: A circular RNA and potential novel biomarker for colorectal cancer
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DOI：
10.1016/j.bbrc.2018.02.036
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发表时间：
2018-02-26
影响因子：
3.1
通讯作者：
Dai, Yinhai
中科院分区：
文献类型：
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作者：
Ji, Wenxin;Qiu, Chunli;Dai, Yinhai
circRNA在多种癌症中差异表达，调节其发生和进展，可作为新的生物标志物用于肿瘤等某些疾病的诊断。然而，circRNA与结直肠癌（CRC）之间的相关性和作用机制仍不清楚。在本研究中，我们利用qRT-PCR检测了CRC患者组织和血清样本中hsa_circ_0001649的表达水平，并对培养的细胞进行了检测。我们发现hsa_circ_0001649在CRC中的表达水平显着低于相应的非肿瘤组织（n = 64，P < 0.01）。我们还测试了 HCTII6 细胞系，观察到类似的结果（n = 15，P < 0.01）。此外，我们对手术前后获得的血清样本进行检测，显示同一患者术后hsa_circ_0001649的表达水平显着上调（n = 18，P < 0.01）。此外，我们分析了临床病理数据与hsa_circ_0001649表达水平的相关性，发现hsa_circ_0001649表达水平与病理分化密切相关（P = 0.037），结果还说明hsa_circ_0001649表达水平与年龄、性别、TMN分期、淋巴结转移、CEA、CA19-9、 CA-724 水平。受试者工作特征 (ROC) 曲线下面积为 0.857。总之，本研究表明 hsa_circ_0001649 的表达水平在 CRC 中下调，可以将其用作 CRC 特异性和灵敏检查的新生物标志物。 (C) 2018 Elsevier Inc. 保留所有权利。
The circRNAs are differentially expressed in a wide range of cancers in regulating their initiation and progression, and could be used to make a diagnosis for some diseases like tumor as a new biomarker. However, the correlation and the mechanism of action between circRNAs and colorectal cancer (CRC) are still unclear. In this study, by using qRT-PCRs, we detected the expression level of hsa_circ_0001649 in tissue and serum samples from CRC patients, and the cultured cell has been detected. We found that the hsa_circ_0001649 in CRC is significantly lower than the expression level of correspondent nontumorous tissues (n = 64, P < 0.01). We also tested the HCTII6 cell lines, and the similar result is observed (n = 15, P< 0.01). Moreover, we detected the serum samples obtained before and after surgery, showing significantly the expression level of hsa_circ_0001649 in the same patient is up-regulated after surgery (n = 18, P < 0.01). Besides, we analyzed the correlation between clinicopathological date and the expression level of hsa_circ_0001649, we found that hsa_circ_0001649 expression level is closely associated with pathological differentiation (P = 0.037), and the result also illustrated that the expression level of hsa_circ_0001649 is no direct correlation with age, gender, TMN stage, lymphatic metastasis, CEA, CA19-9, and CA-724 levels. The area under the receiver operating characteristic (ROC) curve was 0.857. In conclusion, this study showed that the expression level of hsa_circ_0001649 was down regulated in CRC and could use it as a new biomarker for specific and sensitive inspection of CRC. (C) 2018 Elsevier Inc. All rights reserved.