Effects of consecutive monoculture of Pseudostellaria heterophylla on soil fungal community as determined by pyrosequencing.
Effects of consecutive monoculture of Pseudostellaria heterophylla on soil fungal community as determined by pyrosequencing.
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DOI：
10.1038/srep26601
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发表时间：
2016-05-24
影响因子：
4.6
通讯作者：
Lin W
中科院分区：
文献类型：
--
作者：
Wu L;Chen J;Wu H;Wang J;Wu Y;Lin S;Khan MU;Zhang Z;Lin W
连续单一栽培下，太子参的生物量和品质显着下降。在本研究中，进行了为期三年的田间试验，以确定长期单一种植太子参所引起的典型生长抑制效应。使用深度焦磷酸测序来检查单一栽培三年梯度下土壤真菌群落结构和组成的变化。结果显示，新种植的地块与两年、三年单一种植的地块之间存在明显的区别。两年和三年单一栽培土壤的香农和辛普森多样性指数显着高于新种植土壤。连续单一栽培该植物导致镰刀菌属、丝孢霉属、漆斑菌属和单霉菌属等相对丰度显着增加，而青霉属相对丰度显着下降。定量 PCR 分析证实，尖孢镰刀菌（Fusarium oxysporum）显着增加，尖镰孢是一种已知会引起太子参枯萎病和腐烂病的病原体。此外，与根际中发现的比例相似的酚酸混合物可以促进病原性尖孢镰刀菌的菌丝生长。总体而言，这项研究表明，连续单一栽培太子参可以改变根际真菌群落，包括以牺牲植物有益真菌为代价来富集宿主特异性致病真菌。
Under consecutive monoculture, the biomass and quality of Pseudostellaria heterophylla declines significantly. In this study, a three-year field experiment was conducted to identify typical growth inhibition effects caused by extended monoculturing of P. heterophylla. Deep pyrosequencing was used to examine changes in the structure and composition of soil fungal community along a three-year gradient of monoculture. The results revealed a distinct separation between the newly planted plot and the two-year, three-year monocultured plots. The Shannon and Simpson diversity indices were significantly higher in the two-year and three-year monoculture soils than in the newly planted soil. Consecutive monoculture of this plant led to a significant increase in relative abundance of Fusarium, Trichocladium and Myrothecium and Simplicillium, etc., but a significant decrease in the relative abundance of Penicillium. Quantitative PCR analysis confirmed a significant increase in Fusarium oxysporum, an agent known to cause wilt and rot disease of P. heterophylla. Furthermore, phenolic acid mixture at a ratio similar to that found in the rhizosphere could promote mycelial growth of pathogenic F. oxysporum. Overall, this study demonstrated that consecutive monoculture of P. heterophylla can alter the fungal community in the rhizosphere, including enrichment of host-specific pathogenic fungi at the expense of plant-beneficial fungi.