On the lattice Boltzmann method simulation of a two-phase flow bioreactor for artificially grown cartilage cells.
On the lattice Boltzmann method simulation of a two-phase flow bioreactor for artificially grown cartilage cells.
复制标题
人工生长软骨细胞两相流生物反应器的格子玻尔兹曼方法模拟。
DOI：
10.1016/j.jbiomech.2008.09.034
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发表时间：
2008
影响因子：
2.4
通讯作者：
Thomas Becker
中科院分区：
文献类型：
--
作者：
Mohamed Hussein;S. Esterl;Ralf Pörtner;K. Wiegandt;Thomas Becker
随着软骨组织修复和移植需求的日益增长，工程化软骨细胞已成为一种有前景的解决方案。为人工培养的软骨细胞建造了几个生物反应器。在这项工作中，最近设计的流动床生物反应器进行了数值研究，并与实验结果进行了比较。采用格子Boltzmann方法模拟了生物反应器内的流场。流动由两个阶段组成，即液体成分(营养供应)和气体成分(氧气供应)。流场用多相格子Boltzmann方法模拟，电池活性用Michaelis-Menten动力学模拟。营养阶段出口处的氧气扩散水平被用作数值和实验结果之间的评估过程，报告了使用所提出的模型完全模拟此类生物反应器的可能性，尽管极大地节省了时间和金钱。剪切应力和压力分布也与已发表的人类软骨负荷测量结果进行比较，以估计生物反应器和人类膝关节之间的动态相似性。经过1h的测量，预测值与实验值的变化趋势一致，误差为7%。剪应力水平比人类低10-11个数量级，压力分布也低一个数量级。
Owing to the growing demand of cartilage tissue repair and transplants, engineered cartilage cells have emerged as a prospective solution. Several bioreactors were built for artificially grown cartilage cells. In this work, a recently designed flow bed bioreactor is numerically investigated and compared with experimental results. The flow field inside the bioreactor was modelled using the lattice Boltzmann method. The flow consists of two phases which are the liquid component (nutrition supply) and gas component (oxygen supply). The flow field is simulated using the multi-phase lattice Boltzmann method, whilst the cell activity is modelled using Michaelis–Menten kinetics. The oxygen diffusion level at the exit of the nutrition phase is used as an evaluation process between the numerical and experimental results reporting the possibility of using the proposed model to fully simulate such bioreactors, though greatly saving time and money. Shear stress and pressure distributions are as well compared with published human cartilage load measurements to estimate the dynamic similarity between the bioreactor and the human knee. The predicted oxygen levels proved consistent trends with the experimental work with a 7% difference after 1h measuring time. The shear stress levels recorded 10–11 orders of magnitude lower than in humans and also one order of magnitude lower in the pressure distribution.