The Bacillus subtilis rec-assay:: a powerful tool for the detection of genotoxic substances in the water environment.: Prospect for assessing potential impact of pollutants from stabilized wastes
The Bacillus subtilis rec-assay:: a powerful tool for the detection of genotoxic substances in the water environment.: Prospect for assessing potential impact of pollutants from stabilized wastes
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DOI：
10.1016/s0956-053x(01)00071-x
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发表时间：
2002-01-01
期刊：
影响因子：
8.1
通讯作者：
Shimizu, Y
中科院分区：
文献类型：
--
作者：
Takigami, H;Matsui, S;Shimizu, Y
枯草芽孢杆菌re-assay是专门为检测环境水样中的遗传毒性而开发的。枯草芽孢杆菌重新测定的基本原理是基于 DNA 修复重组有效菌株及其缺陷菌株的存活率的相对差异，这被解释为基因毒性。这种测定方法非常强大，因为与其他细菌突变测定相比，它对于检测高度污染的水中的诱变剂具有更高的灵敏度。使用 XAD-2 树脂对各种环境水中的疏水组分进行分离和分析，目标是检测有机遗传毒性物质。其中大部分检测到了遗传毒性反应，这表明公共水体中存在许多具有遗传毒性的未知微污染物。从处理过的城市固体废物（MSW）垃圾填埋场渗滤液中也检测到了积极的反应。处理后的废水中残留的遗传毒性表明，遗传毒性微污染物可能会通过传统的水处理工艺，例如活性污泥处理工艺。如果没有适当控制废物质量和填埋设施，废物填埋场可能会成为严重污染源。 (C) 2002 Elsevier Science Ltd. 保留所有权利。
The Bacillus subtilis rec-assay has been specially developed to detect genotoxicity in environmental water samples. The rationale of the B. subtilis rec-assay is based on the relative difference of survival of a DNA repair-recombination proficient strain and its deficient strain, which is interpreted as genotoxicity. This assay method can be very powerful in that it has higher sensitivity for the detection of mutagens in highly polluted waters than other bacterial mutation assays. Hydrophobic fractions from various environmental waters were fractionated by using XAD-2 resins and assayed, targeting the detection of organic genotoxic substances. Genotoxic response was detected in most of them, which revealed that many unknown micropollutants with genotoxicity occur in public water bodies. Positive response was also detected from a treated municipal solid waste (MSW) landfill leachate. Genotoxicity remaining in the treated effluent suggests that genotoxic micropollutants may pass through conventional water treatment processes such as activated sludge treatment process. Without proper control of waste quality and landfill facilities, waste landfill could be a heavy pollution source. (C) 2002 Elsevier Science Ltd. All rights reserved.