Identification of cartilage progenitor cells in the adult ear perichondrium: utilization for cartilage reconstruction
Identification of cartilage progenitor cells in the adult ear perichondrium: utilization for cartilage reconstruction
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DOI：
10.1038/labinvest.3700409
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发表时间：
2006-05-01
影响因子：
5
通讯作者：
Suzuki, S
中科院分区：
文献类型：
--
作者：
Togo, T;Utani, A;Suzuki, S
对于软骨重建，仍然难以获得足够体积的软骨并长时间保持其功能表型。利用组织干细胞是克服这些困难的一种方法。我们通过5-溴-2 '-脱氧尿苷标记、克隆形成和分化分析，在成年兔耳软骨膜中发现了软骨祖细胞。在软骨膜中发现了长期标记保留细胞。使用剥离器机械分离来自软骨膜的细胞，即软骨膜细胞，并维持在含有10%FCS的D-MEM/F-12培养基中。它们比软骨中的软骨细胞增殖更活跃。软骨周细胞在分化诱导液中可分化为脂肪细胞和骨细胞。对于体内软骨重建，将软骨周细胞接种在胶原海绵支架上并植入裸鼠体内。4周后，软骨周细胞的复合物产生与软骨细胞相同重量的软骨组织。RT-PCR和免疫组化检测显示，它们产生糖胺聚糖和II型胶原。相反，兔骨髓间充质干细胞作为对照，在植入研究中可以再生明显小于软骨周细胞的软骨。基于这些发现，我们提出，软骨膜含有组织祖细胞是用于重建软骨和新的治疗模式的潜在候选人之一。
For cartilage reconstruction, it is still difficult to obtain a sufficient volume of cartilage and to maintain its functional phenotype for a long period. Utilizing tissue stem cells is one approach to overcome such difficulties. We show here the presence of cartilage progenitor cells in the ear perichondrium of adult rabbits by 5-bromo-2'-deoxyuridine labeling, clonogenicity, and differentiation analyses. Long-term label-retaining cells were demonstrated in the perichondrium. Cells from the perichondrium, that is, perichondrocytes were mechanically isolated using a raspatory and maintained in D-MEM/F-12 medium with 10% FCS. They proliferated more vigorously than chondrocytes from the cartilage. Perichondrocytes could differentiate into adipocytes as well as osteocytes in differentiation induction medium. For cartilage reconstruction in vivo, perichondrocytes were seeded on collagen sponge scaffolds and implanted in nude mice. After 4 weeks, the composites with perichondrocytes generated the same weight of cartilaginous tissue as those with chondrocytes. They produced glycosaminoglycan and type II collagen as shown by RT-PCR and immunohistochemical examination. On the contrary, rabbit bone marrow mesenchymal stem cells used as control could regenerate significantly smaller cartilage than perichondrocytes in the implant study. Based on these findings, we propose that the perichondrium containing tissue progenitor cells is one of the potential candidates for use in reconstructing cartilage and new therapeutic modalities.