Model for the prediction of mechanical asphyxia as the cause of death based on four biological indexes in human cardiac tissue
Model for the prediction of mechanical asphyxia as the cause of death based on four biological indexes in human cardiac tissue
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DOI：
10.1016/j.scijus.2021.02.003
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发表时间：
2021-05-10
影响因子：
1.9
通讯作者：
Chen, Long
中科院分区：
文献类型：
--
作者：
Han, Liujun;Li, Wencan;Chen, Long
对于法医病理学家来说，确定机械性窒息是死因一直是个难题，特别是当窒息迹象在尸体上不明显时。目前，仅依靠对尸体的体格检查，病理学家在进行死因评估时必须谨慎。在之前的一项研究中，我们在因机械性窒息死亡的尸体与因颅脑损伤、失血性休克或其他原因死亡的尸体的心脏组织中筛选了四种生物标志物——双特异性磷酸酶1 (DUSP1)、钾电压门控通道亚家族J成员2 (KCNJ2)、miR-122和miR-3185。无论年龄、环境温度和死后间隔如何，标记物的表达与机械性窒息死亡相关。然而，单一的生物学指标并不能成为鉴定死因的准确依据。在本研究中，生成了四个指标的 Delta Cq 值的受试者工作特征曲线。根据曲线下面积判断指标的诊断准确性（DUSP1：0.773，KCNJ2：0.775，miR-122：0.667，miR-3185：0.801）。最后生成列线图，并进行单盲实验验证机械性窒息的死亡原因。
Determination of mechanical asphyxia as the cause of death has always been difficult for forensic pathologists, particularly when signs of asphyxia are not obvious on the body. Currently, depending on only physical examination of corpses, pathologists must be cautious when making cause-of-death appraisals. In a previous study, four biomarkers-dual-specificity phosphatase 1 (DUSP1), potassium voltage-gated channel subfamily J member 2 (KCNJ2), miR-122, and miR-3185-were screened in human cardiac tissue from cadavers that died from mechanical asphyxia compared with those that died from craniocerebral injury, hemorrhagic shock, or other causes. Expression of the markers correlated with death from mechanical asphyxia regardless of age, environmental temperature, and postmortem interval. However, a single biological index is not an accurate basis for the identification of the cause of death. In this study, receiver operating characteristic curves of the Delta Cq values of the four indexes were generated. The diagnostic accuracy of the indexes was judged according to their area under the curve (DUSP1: 0.773, KCNJ2: 0.775, miR-122: 0.667, and miR-3185: 0.801). Finally, a nomogram was generated, and single blind experiment was conducted to verify the cause of death of mechanical asphyxia.