Rabbit spermatozoa undergo an acrosome reaction in the presence of glycosaminoglycans
Rabbit spermatozoa undergo an acrosome reaction in the presence of glycosaminoglycans
复制标题
兔精子在糖胺聚糖存在下发生顶体反应
DOI：
--
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
R. Ax
中科院分区：
文献类型：
--
作者：
R. Lenz;M. Bellin;R. Ax
将家兔精液在含有市售糖胺聚糖（GAG）的化学成分确定的培养基中孵育，浓度范围为0.1 - 100 μg/ml。孵育9 h后，对精子进行染色并检查顶体反应程度和活力。有显着的剂量和治疗效果的诱导顶体反应。两种内毒素或治疗组之间的存活率无显著差异。肝素在0.1 ~ 1.0 μg/ml浓度范围内可促进精子顶体反应，而更高浓度则可抑制精子顶体反应。精浆添加到精子培养物中抑制了GAG的刺激作用。用软骨素-4-硫酸酯酶处理软骨素-4-硫酸酯抑制了刺激作用。它的结论是，糖胺酸，女性生殖道的组成部分，可能会促进顶体反应，使成功受精可以发生。
Rabbit-ejaculated spermatozoa were incubated in a chemically defined medium containing comercially available glycosaminoglycans (GAGs) at concentrations ranging from 0.1 to 100 μg/ml. Sperm were stained and examined for the degree of acrosome reaction and viability after 9 h of incubation. There were significant dose and treatment effects of the induction of the acrosome reaction. Viability did not differ significantly betweendoses or treatment. Heparin enhanced the acrosome reaction between concentrations of 0.1 to 1.0 μg/ml, whereas higher levels depressed the percentage of sperm undergoing the acrosome reaction. Seminal plasma added to sperm cultures depressed the stimulatory effect of GAGs. Treatment of chondroitin-4-sulfate with chondro-4-sulfatase prohibited the stimulatory effect. It is concluded that GAGs, components of the female reproductive tract, may promote the acrosome reaction so that successful fertilization can occur.