Spectrum of Aminoglycoside Modifying Enzymes in Gram-Negative Bacteria Causing Human Infections
Spectrum of Aminoglycoside Modifying Enzymes in Gram-Negative Bacteria Causing Human Infections
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DOI：
10.1055/s-0040-1713687
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发表时间：
2020-03-01
影响因子：
1.1
通讯作者：
Sekar, Uma
中科院分区：
文献类型：
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作者：
Aishwarya, Kayanam Vijaya Lalitha;Geetha, Pacha Venkataramana;Sekar, Uma
简介 氨基糖苷类是临床使用的强大广谱抗生素；不幸的是，由于主要由氨基糖苷修饰酶 (AME) 引起的耐药性的出现和分布，它们的使用已减少。 目的 本研究旨在确定革兰氏阴性菌临床分离株中 AME 的不同患病率及其发生模式。本研究还旨在检测革兰氏阳性菌和革兰氏阴性菌中普遍存在的 AME。 材料和方法 本研究共纳入 386 个临床分离株。聚合酶链式反应显示筛选的 AME 患病率 [aac(6 ')-lb, aac(3 ')-I, aac(3 ')-II, aac(3 ')-VI, ant(2 ')-I, ant(4 ')-IIb, aac(3 ')-III, aac(3 ')-IV, aph(2 ')-Ib, aph(2 ')-Ic、aph(2')-Id、aac(6')-Ie-aph(2')-Ia 和 aph(3')-IIIa]。对含有革兰氏阳性菌中流行的任何一种AME的临床分离株进行接合实验[aph(3')-IIIa、aac(6')-Ie-aph(2')-Ia]。结果aac(6')-lb是最流行的AME，其次是aac(3')-I、aph(3')-VI、aac(3')-VI、和aac(3')-II。在我们的研究分离株中未建立 ant (2 ')-I、ant(4 ')-IIb、aac(3 ')-III、aac(3 ')-IV、aph(2 ')-Ib、aph(2 ')-Ic 和 aph(2 ')-Id 等 AME。 aph(3')-IIIa、aac(6')-Ie-aph(2')-Ia-革兰氏阳性菌中遇到的 AME 及其共存率为 19.68%，接合实验揭示了它们通过质粒转移。 结论 这是来自印度的第一份报告，揭示了革兰氏阴性菌中革兰氏阳性菌中经常遇到的 AME 的存在和患病率以及它们在革兰氏阴性菌中的存在。接合质粒。
Introduction Aminoglycosides are formidable broad-spectrum antibiotics used in clinical settings; woefully their usage has been reduced by the emergence and distribution of resistance mainly due to aminoglycoside modifying enzymes (AME).Purpose This study was performed to determine the diverse prevalence of AME and their pattern of occurrence in the clinical isolates of gram-negative bacteria. This study also aimed to detect the presence of AMEs that are prevalent in gram-positive bacteria, among gram negatives.Materials and Methods A total number of 386 clinical isolates were included in this study. Polymerase chain reaction revealed the prevalence rate of AMEs screened [aac(6 ')-lb, aac(3 ')-I, aac(3 ')-II, aac(3 ')-VI, ant(2 ')-I, ant(4 ')-IIb, aac(3 ')-III, aac(3 ')-IV, aph(2 ')-Ib, aph(2 ')-Ic, aph(2 ')-Id, aac (6 ')-Ie- aph(2 ')-Ia, and aph(3 ')-IIIa]. Conjugation experiment was performed for the clinical isolates which harbored any one of the AME which was prevalent in gram-positive bacteria [aph(3 ')-IIIa, aac(6 ')-Ie-aph(2 ')-Ia].Results aac(6 ')-lb is the most prevalent AME, followed by aac(3 ')-I, aph(3 ')-VI, aac(3 ')-VI, and aac(3 ')-II. The AMEs such as ant (2 ')-I, ant(4 ')-IIb, aac(3 ')-III, aac(3 ')-IV, aph(2 ')-Ib, aph(2 ')-Ic, and aph(2 ')-Id were not established in our study isolates. The rate of prevalence of aph(3 ')-IIIa, aac(6 ')-Ie-aph(2 ')-Ia-the AMEs encountered in gram-positive and their co-existence was 19.68% and the conjugation experiment revealed their transfer via plasmids.Conclusion This is the first report from India revealing the presence and prevalence of AMEs which are often encountered among gram-positive bacteria in gram negatives and their presence on conjugative plasmids.