Photoactivated composite biomaterial for soft tissue restoration in rodents and in humans.
Photoactivated composite biomaterial for soft tissue restoration in rodents and in humans.
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DOI：
10.1126/scitranslmed.3002331
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发表时间：
2011-07-27
影响因子：
17.1
通讯作者：
Elisseeff JH
中科院分区：
文献类型：
--
作者：
Hillel AT;Unterman S;Nahas Z;Reid B;Coburn JM;Axelman J;Chae JJ;Guo Q;Trow R;Thomas A;Hou Z;Lichtsteiner S;Sutton D;Matheson C;Walker P;David N;Mori S;Taube JM;Elisseeff JH
软组织重建通常需要多次手术，可能会导致疤痕和毁容。面部软组织重建是一个临床挑战，因为即使是轻微的畸形也会严重影响个人的社会和心理功能。因此，我们开发了一种生物合成的软组织替代物，它由聚乙二醇(PEG)和透明质酸(HA)组成，可以在透皮光照射下进行注射和原位光交联。通过调节合成聚合物和生物聚合物的比例，我们可以调整植入物的弹性和体积持久性。在小动物模型中，植入光交联型聚乙二醇-羟基磷灰石显示出剂量依赖关系，即增加聚乙二醇浓度和增强植入体积持久性。与市售的HA注射相比，聚乙二醇HA植入物的平均体积和高度显著增加。植入体积的可逆性是通过注射透明质酸酶实现的。在人类患者中进行的初步临床测试证实了透皮光交联法植入腹部软组织的可行性，尽管在一些材料周围观察到了炎症反应。
Soft tissue reconstruction often requires multiple surgical procedures that can result in scars and disfiguration. Facial soft tissue reconstruction represents a clinical challenge because even subtle deformities can severely affect an individual’s social and psychological function. We therefore developed a biosynthetic soft tissue replacement composed of poly(ethylene glycol) (PEG) and hyaluronic acid (HA) that can be injected and photocrosslinked in situ with transdermal light exposure. Modulating the ratio of synthetic to biological polymer allowed us to tune implant elasticity and volume persistence. In a small-animal model, implanted photocrosslinked PEG-HA showed a dose-dependent relationship between increasing PEG concentration and enhanced implant volume persistence. In direct comparison with commercial HA injections, the PEG-HA implants maintained significantly greater average volumes and heights. Reversibility of the implant volume was achieved with hyaluronidase injection. Pilot clinical testing in human patients confirmed the feasibility of the transdermal photocrosslinking approach for implantation in abdomen soft tissue, although an inflammatory response was observed surrounding some of the materials.