Expression and localization of secreted frizzled-related protein-4 in the rodent ovary: Evidence for selective up-regulation in luteinized granulosa cells
Expression and localization of secreted frizzled-related protein-4 in the rodent ovary: Evidence for selective up-regulation in luteinized granulosa cells
复制标题
DOI：
10.1210/en.2003-0048
复制
发表时间：
2003-10-01
期刊：
影响因子：
4.8
通讯作者：
Richards, JS
中科院分区：
文献类型：
--
作者：
Hsieh, M;Mulders, SM;Richards, JS
分泌卷曲相关蛋白-4 （sFRP-4）属于可溶性蛋白家族，具有卷曲样富含半胱氨酸的结构域，并作为wnt -卷曲（Fz）信号的调节剂。由于几种wnt和Fz在啮齿类动物卵巢卵泡发育的特定阶段表达，因此这些研究旨在评估激素调节的sFRP-4的表达和定位。通过RT-PCR和原位杂交分析发现，在小鼠卵巢中，人绒毛膜促性腺激素给药后，大窦卵泡颗粒细胞中sFRP-4 mRNA的表达上调，黄体中也升高。在去垂体的大鼠卵巢中，人绒毛膜促性腺激素同样诱导了sFRP-4的表达，并进一步被PRL上调。PRL还能刺激培养的黄体化大鼠颗粒细胞分泌sFRP-4蛋白。因此，LH和PRL对sFRP-4的调控可能对Fz-1和Wnt-4的调控很重要，Fz-1已知在排卵卵泡中表达，Wnt-4/Fz-4在黄体中表达。
Secreted frizzled-related protein-4 (sFRP-4) belongs to a family of soluble proteins that have a Frizzled-like cysteine-rich domain and function as modulators of Wnt-Frizzled (Fz) signals. As several Wnts and Fz are expressed at defined stages of follicular development in rodent ovaries, these studies were undertaken to evaluate the hormone-regulated expression and localization of sFRP-4. In the mouse ovary, the expression of sFRP-4 mRNA was up-regulated in granulosa cells of large antral follicles after human chorionic gonadotropin administration and was also elevated in corpora lutea, as determined by RT-PCR and in situ hybridization analyses. In hypophysectomized rat ovaries, sFRP-4 expression was similarly induced by human chorionic gonadotropin and further up-regulated by PRL. PRL also stimulated the secretion of sFRP-4 protein from luteinized rat granulosa cells in culture. Therefore, regulation of sFRP-4 by LH and PRL may be important for modulating Fz-1, which is known to be expressed in periovulatory follicles, and Wnt-4/Fz-4, which are expressed in corpora lutea.