Expression of L-type amino acid transporter 1 (LAT1) and 4F2 heavy chain (4F2hc) in oral squamous cell carcinoma and its precusor lesions.
Expression of L-type amino acid transporter 1 (LAT1) and 4F2 heavy chain (4F2hc) in oral squamous cell carcinoma and its precusor lesions.
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发表时间：
2004-05
影响因子：
2
通讯作者：
Do Kyung Kim;Sang‐Gun Ahn;Joo-Cheol Park;Y. Kanai;H. Endou;J. Yoon
中科院分区：
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作者：
Do Kyung Kim;Sang‐Gun Ahn;Joo-Cheol Park;Y. Kanai;H. Endou;J. Yoon
氨基酸转运蛋白在向细胞提供有机营养物质方面起着重要作用。通过检测l型氨基酸转运蛋白1 （LAT1）及其亚基4F2重链（4F2hc）在口腔正常黏膜（ONM）、口腔癌前病变（OPL）和口腔鳞状细胞癌（OSCC）中的表达，确定这些转运蛋白在口腔正常黏膜（ONM）、口腔癌前病变（OPL）和口腔鳞状细胞癌（OSCC）中的表达变化。材料与方法采用免疫组化染色法检测福尔马林固定、石蜡包埋的ONM、OPL或OSCC切片，检测L4T1和4F2hc蛋白。结果LAT1和4F2hc的表达从ONM到增生，再到发育不良病变和OSCC逐渐升高。特别是，LAT1可能是比4F2hc更特异的肿瘤进展指标。结论LAT1和4F2hc可能在口腔多步骤癌变的早期阶段起重要作用。此外，特异性抑制LAT1和4F2hc可能是抑制口腔癌进展的新原理。
BACKGROUND Amino acid transporters play an important role in supplying organic nutrients to cells. The expression of L-type amino acid transporter 1 (LAT1) and its subunit 4F2 heavy chain (4F2hc) was evaluated to determine the alterations of these transporters in oral normal mucosa (ONM), oral precancerous lesion (OPL) and oral squamous cell carcinoma (OSCC). MATERIALS AND METHODS Sections from formalin-fixed, paraffin-embedded samples of ONM, OPL or OSCC were examined using immunohistochemical staining to detect L4T1 and 4F2hc proteins. RESULTS The LAT1 and 4F2hc expression increased progressively from ONM to hyperplastic and to dysplastic lesions and OSCC. In particular, LAT1 may be a more specific indicator of tumor progression than 4F2hc. CONCLUSION LAT1 and 4F2hc may have an important role in the early stages of multistep oral carcinogenesis. In addition, the specific inhibition of LAT1 and 4F2hc might be a new rationale to suppress oral cancer progression.