Variation in commercial rodent diets induces disparate molecular and physiological changes in the mouse uterus
Variation in commercial rodent diets induces disparate molecular and physiological changes in the mouse uterus
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DOI：
10.1073/pnas.0501632102
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发表时间：
2005-07-12
影响因子：
11.1
通讯作者：
Dey, SK
中科院分区：
文献类型：
--
作者：
Wang, HB;Tranguch, S;Dey, SK
虽然卵巢雌激素，雌二醇-17 β，是正常生殖功能的关键调节剂，具有雌激素样活性的天然和合成化合物可以进一步影响生殖功能。植物来源的植物雌激素特别受到关注，因为相关的健康益处。然而，通过商业啮齿动物饮食对子宫生物学和早期妊娠在分子水平上的有益和/或有害影响的植物雌激素消费的全面理解仍然在很大程度上未被探索。使用多种方法，我们在这里证明，暴露的成年雌性小鼠的商业啮齿类动物的饮食具有较高的植物雌激素水平促进子宫生长的存在或不存在的卵巢雌激素，改变子宫表达的雌激素反应基因，并提前植入的时间与饮食相比，植物雌激素水平较低。商业啮齿动物饮食中植物雌激素含量的变化，无论是品牌内部还是品牌之间，都会影响实验结果，这一发现强调了这项调查的重要性，并对使用啮齿动物作为动物模型的研究人员提出了警告。
Although ovarian estrogen, estradiol-17 beta, is a key modulator of normal reproductive functions, natural and synthetic compounds with estrogen-like activities can further influence reproductive functions. Plant-derived phytoestrogens specifically have received much attention because of associated health benefits. However, a comprehensive understanding of the beneficial and/or detrimental impacts of phytoestrogen consumption through commercial rodent diets on uterine biology and early pregnancy at the molecular level remains largely unexplored. Using multiple approaches, we demonstrate here that exposure of adult female mice to a commercial rodent diet with higher phytoestrogen levels facilitates uterine growth in the presence or absence of ovarian estrogen, alters uterine expression of estrogen-responsive genes, and advances the timing of implantation compared with a diet with lower phytoestrogen levels. The finding that variability in phytoestrogen content in commercial rodent diets, both within and between brands, influences experimental results stresses the importance of this investigation and raises caution for investigators using rodents as animal models.