THE POSTTRANSLATIONAL MODIFICATION OF PHOSPHOGLUCOMUTASE IS REGULATED BY GALACTOSE INDUCTION AND GLUCOSE REPRESSION IN SACCHAROMYCES-CEREVISIAE
THE POSTTRANSLATIONAL MODIFICATION OF PHOSPHOGLUCOMUTASE IS REGULATED BY GALACTOSE INDUCTION AND GLUCOSE REPRESSION IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1128/jb.177.11.3087-3094.1995
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发表时间：
1995-06-01
影响因子：
3.2
通讯作者：
BEDWELL, DM
中科院分区：
文献类型：
--
作者：
FU, LW;BOUNELIS, P;BEDWELL, DM
葡萄糖磷酸变位酶在碳水化合物代谢的关键点发挥作用。在本文中，我们表明，酵母磷酸葡萄糖变位酶的主要亚型，由GAL 5（PGM 2）基因编码的合成，调节的方式是不同于先前描述的其他酶参与半乳糖代谢的酵母酿酒酵母。当培养物经历葡萄糖耗竭或热休克时，这种异构体的积累增加了4 - 6倍。然而，热休克诱导没有发生，除非细胞在葡萄糖阻遏。这种非累加性的表达增加表明，控制GAL 5基因的热休克诱导和葡萄糖阻遏的调节机制在功能上是相关的。我们以前证明，磷酸葡萄糖变位酶是由翻译后葡萄糖磷酸化反应，我们现在表明，这种翻译后修饰，如磷酸葡萄糖变位酶表达本身，也受到半乳糖诱导和葡萄糖阻遏，最后，没有证据表明，葡萄糖磷酸化磷酸葡萄糖变位酶改变其酶活性的条件下检查。
The enzyme phosphoglucomutase functions at a key point in carbohydrate metabolism. In this paper, we show that the synthesis of the major isoform of yeast phosphoglucomutase, encoded by the GAL5 (PGM2) gene, is regulated in a manner that is distinct from that previously described for other enzymes involved in galactose metabolism in the yeast Saccharomyces cerevisiae. Accumulation of this isoform increased four- to sixfold when the culture experienced either glucose depletion or heat shock. However, heat shock induction did not occur unless the cells were under glucose repression. This nonadditive increase in expression suggests that the regulatory mechanisms controlling the heat shock induction and glucose repression of the GAL5 gene are functionally related. We previously demonstrated that phosphoglucomutase is modified by a posttranslational Glc-phosphorylation reaction, We now show that this posttranslational modification, like phosphoglucomutase expression itself, is also regulated by galactose induction and glucose repression, Finally, no evidence was found to indicate that the Glc-phosphorylation of phosphoglucomutase alters its enzymatic activity under the conditions examined.