CTLA-4 exon 1 +49 polymorphism alone and in a haplotype with -318 promoter polymorphism may confer susceptibility to chronic HBV infection in Chinese Han patients
CTLA-4 exon 1 +49 polymorphism alone and in a haplotype with -318 promoter polymorphism may confer susceptibility to chronic HBV infection in Chinese Han patients
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DOI：
10.1007/s11033-010-0660-7
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发表时间：
2011-11-01
影响因子：
2.8
通讯作者：
Xing, Fanfan
中科院分区：
文献类型：
--
作者：
Duan, Shaoqiong;Zhang, Guoyu;Xing, Fanfan
细胞毒性T淋巴细胞相关抗原4（CTLA-4）在调节T细胞活化中起着关键作用，而T细胞活化对B型肝炎病毒（HBV）感染的转归至关重要。CTLA-4的表达和功能可能受基因多态性的影响。本研究旨在探讨CTLA-4基因多态性对中国汉族慢性HBV感染者疾病易感性的影响。采用聚合酶链反应扩增CTLA-4基因+49 A/G和-318 C/T多态性，然后进行限制性片段长度多态性分析。慢性HBV感染者CTLA-4 + 49 A/G多态性AA基因型和A等位基因频率较高。单倍型+49A-318C在患者中的表达明显高于对照组（P < 0.001），而单倍型+49G-318C在患者中的表达低于对照组（P = 0.006）。HBeAg阳性的慢性B型肝炎患者ALT水平较低者+49 GG基因型频率更高（P = 0.009），+49 A等位基因频率更低（P = 0.012）。提示CTLA-4 +49 A/G多态性可能与中国汉族人群慢性HBV感染易感性有关。
Cytotoxic T-lymphocyte-associated antigen 4 (CTLA-4) plays a pivotal role in regulating T cell activation, which is believably critical for the outcome of hepatitis B virus (HBV) infection. The expression and function of CTLA-4 may be affected by gene polymorphisms. This study investigated the influence of CTLA-4 polymorphisms on disease susceptibility in Chinese Han patients with chronic HBV infection. CTLA-4 +49A/G and -318C/T polymorphisms were evaluated by DNA amplification with polymerase chain reaction followed by the restriction fragment length polymorphism analysis. The patients with chronic HBV infection had higher frequencies of genotype AA and allele A of CTLA-4 +49A/G polymorphism. The haplotype +49A-318C was significantly over-represented (P < 0.001) and haplotype +49G-318C under-represented (P = 0.006) in the patients. The +49GG genotype was more frequent (P = 0.009) and +49A allele was less frequent in patients with lower ALT levels (P = 0.012) in HBeAg positive chronic hepatitis B. It is indicated that CTLA-4 +49A/G polymorphism alone and in a haplotype with -318C allele may confer susceptibility to chronic HBV infection in Chinese Han patients.