The roof plate regulates cerebellar cell-type specification and proliferation
The roof plate regulates cerebellar cell-type specification and proliferation
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DOI：
10.1242/dev.02441
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发表时间：
2006-08-01
期刊：
影响因子：
4.6
通讯作者：
Millen, Kathleen J.
中科院分区：
文献类型：
--
作者：
Chizhikov, Victor V.;Lindgren, Anne G.;Millen, Kathleen J.
在胚胎发育过程中，峡部组织者，一个被充分描述的位于中后脑交界处的信号中心，沿着神经管的前/后轴沿着建立小脑区域。在早期小脑原基内指定不同种群的机制还不太明确。使用一个新开发的早期小脑原基的基因表达图谱，我们表明，分泌信号从菱形1顶板是必要的和足够的规格相邻的小脑菱形唇及其衍生物的命运。令人惊讶的是，我们发现，顶板是不是绝对需要的初始规格更远的小脑细胞的命运，而是调节祖细胞增殖和小脑原基内的细胞位置。因此，除了峡部，顶板代表了控制小脑图案的多个方面的重要信号中心。
During embryogenesis, the isthmic organizer, a well-described signaling center at the junction of the mid-hindbrain, establishes the cerebellar territory along the anterior/posterior axis of the neural tube. Mechanisms specifying distinct populations within the early cerebellar anlage are less defined. Using a newly developed gene expression map of the early cerebellar anlage, we demonstrate that secreted signals from the rhombomere 1 roof plate are both necessary and sufficient for specification of the adjacent cerebellar rhombic lip and its derivative fates. Surprisingly, we show that the roof plate is not absolutely required for initial specification of more distal cerebellar cell fates, but rather regulates progenitor proliferation and cell position within the cerebellar anlage. Thus, in addition to the isthmus, the roof plate represents an important signaling center controlling multiple aspects of cerebellar patterning.