Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner
Icariin induces osteogenic differentiation in vitro in a BMP- and Runx2-dependent manner
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DOI：
10.1016/j.bbrc.2008.02.054
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发表时间：
2008-05-02
影响因子：
3.1
通讯作者：
Nagamune, Teruyuki
中科院分区：
文献类型：
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作者：
Zhao, Jiyuan;Ohba, Shinsuke;Nagamune, Teruyuki
为了利用骨再生医学有效治疗骨病，迫切需要开发安全、廉价、能有效诱导骨形成的药物。本文研究了淫羊藿（Epimedium pubescens）的主要活性成分淫羊藿苷的成骨作用。淫羊藿苷诱导成骨前MC3T3-E1细胞和小鼠原代成骨细胞成骨分化。淫羊藿苷上调成骨细胞标记基因runt相关转录因子2 （Runx2）和dna结合抑制因子1 (Id-1) mRNA表达水平。引入Smad6或显性阴性Runx2以及Noggin治疗可抑制成骨作用。此外，淫羊藿苷诱导骨形态发生蛋白(BMP)-4的mRNA表达。这些数据表明淫羊藿苷通过诱导Runx2的表达、BMP-4的产生和BMP信号的激活来发挥其强大的成骨作用。淫羊藿苷极低的成本和丰富的含量使其对骨再生医学具有吸引力。(C) 2008爱思唯尔公司版权所有。
To effectively treat bone diseases using bone regenerative medicine, there is an urgent need to develop safe cheap drugs that can potently induce bone formation. Here, we demonstrate the osteogenic effect of icariin, the main active compound of Epimedium pubescens. Icariin induced osteogenic differentiation in pre-osteoblastic MC3T3-E1 cells and mouse primary osteoblasts. Icariin upregulated the mRNA expression levels of the osteoblast marker genes runt-related transcription factor 2 (Runx2) and inhibitor of DNA-binding 1 (Id-1). The osteogenic effect was inhibited by the introduction of Smad6 or dominant-negative Runx2, as well as Noggin treatment. Furthermore, icariin induced the mRNA expression of bone morphogenetic protein (BMP)-4. These data suggest that icariin exerts its potent osteogenic effect through induction of Runx2 expression, production of BMP-4 and activation of BMP signaling. The extremely low cost of icariin and its high abundance make it appealing for bone regenerative medicine. (C) 2008 Elsevier Inc. All rights reserved.