Expression of Tumor Necrosis Factor-&agr;, Interleukin-6, and Interferon-&ggr; in Spontaneous Chronic Pancreatitis in the WBN/Kob Rat
Expression of Tumor Necrosis Factor-&agr;, Interleukin-6, and Interferon-&ggr; in Spontaneous Chronic Pancreatitis in the WBN/Kob Rat
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肿瘤坏死因子的表达-
DOI：
--
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
N. Sawabu
中科院分区：
文献类型：
--
作者：
M. Xie;Y. Motoo;S. Su;N. Sawabu
为了阐明细胞因子在慢性胰腺炎（CP）中的病理生理学意义，我们分析了WBN/Kob大鼠自发性CP发病和进展中各种细胞因子的组织表达。4周龄雄性WBN/Kob大鼠饲喂特殊颗粒饲料（MB-3）20周，每4周处死6只大鼠。病理上，CP发生在12周，此后进展。炎症和纤维化分别在12周和16周达到高峰。通过半定量逆转录聚合酶链反应，肿瘤坏死因子（TNF）-，白细胞介素（IL）-6，和干扰素（IFN）-mRNA的表达分别在8周，12周和16周达到峰值。&agr;&ggr;免疫组化显示IL-6在浸润的炎性细胞和血管内皮细胞中表达，而TNF-α在腺泡和浸润细胞中表达。&agr; IFN-γ定位于腺泡、浸润和导管细胞，其表达强度与纤维化、Ⅲ型胶原和平滑肌肌动蛋白的表达强度呈显著相关。&ggr;&agr;原位杂交结果与RT-PCR结果一致。这些结果表明，TNF-α和IL-6的组织表达参与了胰腺炎的发病，IFN-α的表达与CP的进展有关。&agr;&ggr;
To clarify the pathophysiological significance of cytokines in chronic pancreatitis (CP), we analyzed tissue expressions of various cytokines in the onset and progression of spontaneous CP in the WBN/Kob rat. Four-week-old male WBN/Kob rats were fed a special pellet diet (MB-3) for 20 weeks, and 6 rats were killed every 4 weeks. Pathologically, CP occurred at 12 weeks and progressed thereafter. The inflammation and fibrosis peaked at 12 and 16 weeks, respectively. By semiquantitative reverse transcription-polymerase chain reaction, the expression of tumor necrosis factor (TNF)-&agr;, interleukin (IL)-6, and interferon (IFN)-&ggr; mRNAs peaked at 8, 12, and 16 weeks, respectively. Immunohistochemistry showed IL-6 expression in infiltrating inflammatory cells and vascular endothelial cells, whereas TNF-&agr; was expressed in both acinar and infiltrating cells. IFN-&ggr; was localized to acinar, infiltrating and ductal cells, and its expression intensity showed significant correlation with those of fibrosis, type III collagen and &agr;-smooth muscle actin. The in situ hybridization results were consistent with the RT-PCR data. These results suggest that tissue expressions of TNF-&agr; and IL-6 are involved in the onset of pancreatitis and that IFN-&ggr; expression is related to the progression of CP.
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影响因子：
24.5
作者：
E. Riesle;H. Friess;L. Zhao;Markus Wagner;W. Uhl;K. Baczako;Leslie I. Gold;M. Korc;M. Büchle
通讯作者：
E. Riesle;H. Friess;L. Zhao;Markus Wagner;W. Uhl;K. Baczako;Leslie I. Gold;M. Korc;M. Büchle
DOI：
10.1111/j.1530-0277.1999.tb04202.x
发表时间：
1999-05-01
影响因子：
3.2
作者：
Tsukamoto, H
通讯作者：
Tsukamoto, H
用抗-IFN-γ治疗IFN-γ转基因小鼠揭示了成年胰腺的重塑能力。
影响因子：
7.7
作者：
Gu,D;Molony,L;Krahl,T;Sarvetnick,N
通讯作者：
Sarvetnick,N
肿瘤坏死因子-α 诱导长期培养的肝细胞凋亡。
DOI：
--
发表时间：
1996
期刊：
The American journal of pathology.
影响因子：
--
作者：
Bour,ES;Ward,LK;Cornman,GA;Isom,HC
通讯作者：
Isom,HC