Mutations in an upstream regulatory sequence that increase expression of the bacteriophage T4 lysozyme gene.
Mutations in an upstream regulatory sequence that increase expression of the bacteriophage T4 lysozyme gene.
复制标题
上游调控序列的突变会增加噬菌体 T4 溶菌酶基因的表达。
DOI：
10.1128/jb.169.10.4630-4636.1987
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发表时间：
1987
影响因子：
3.2
通讯作者：
Poteete,AR
中科院分区：
文献类型：
--
作者：
Knight,JA;Hardy,LW;Rennell,D;Herrick,D;Poteete,AR
构建了一个携带噬菌体T4溶菌酶基因(e)的P22杂交噬菌体，以及溶菌酶基因上游的T4序列。将琥珀突变引入到杂交噬菌体的基因e中，并测试所产生的突变噬菌体在琥珀抑制菌株上形成斑块的能力。在紫外诱变后，能够在琥珀色抑制因子上形成斑块而不抑制亲本琥珀色突变噬菌体的逆转噬菌体被分离出来。通过基因检测鉴定了次生假回复性。对4个抑制次要位点突变进行了定位和测序。他们发现它们由基因e上游的小序列改变组成，所有这些改变都倾向于破坏转录物中潜在的碱基配对相互作用的稳定性。当将这些突变引入野生型杂交噬菌体时，可以增加溶菌酶的表达，但对溶菌酶基因的转录几乎没有影响。
A P22 hybrid phage bearing the bacteriophage T4 lysozyme gene (e), as well as T4 sequences upstream from the lysozyme gene, was constructed. Amber mutations were introduced into gene e in the hybrid phage, and the resulting mutant phages were tested for the ability to form plaques on amber suppressor strains. Revertant phages that were able to form plaques on amber suppressors that did not suppress the parent amber mutant phages were isolated following UV mutagenesis. Secondary site pseudorevertants were identified among the revertants by a genetic test. Four of the suppressing secondary site mutations were mapped and sequenced. They were found to consist of small sequence alterations immediately upstream from gene e, all of which would tend to destabilize potential base-pairing interactions in the transcript. The mutations were shown to increase lysozyme expression when introduced into an otherwise wild-type hybrid phage, but were found to have little effect on transcription of the lysozyme gene.