New map of bacteriophage lambda DNA
New map of bacteriophage lambda DNA
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噬菌体 lambda DNA 新图谱
DOI：
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发表时间：
1980
影响因子：
5.4
通讯作者：
Frederick R. Blattner
中科院分区：
文献类型：
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作者：
Donna L. Daniels;R. Jeffrey;De;Wet;Frederick R. Blattner
构建了噬菌体Lambda的图谱，包括由12种不同的限制性内切酶组成的所有41个切点的准确位置。测量了来自单一、多个和部分酶消化的100多个片段，对照已知序列的DNA分子校准的标准。对数据进行最小二乘分析以分配地图坐标。在任何情况下，从MAP预测的片段大小与片段的测量结果的差异都不会超过+/-5%。这种低错误率在所有碎片大小范围内都是一致的。全长为49,133个核苷酸对。这可能精确到500个碱基对以内。
A map of bacteriophage lambda was constructed, including accurate positions for all 41 cut sites made by 12 different restriction enzymes. Over 100 fragments from single, multiple, and partial enzyme digestions were measured versus standards that were calibrated with respect to DNA molecules of known sequence. The data were subjected to least-squares analysis to assign map coordinates. In no case did a fragment size predicted from the map differ from the measurement of the fragment by more than +/- 5%. This low error rate was consistent in all size ranges of fragments. The total length of lambda was calculated as 49,133 nucleotide pairs. This probably is accurate to within 500 base pairs.