Instrument design and characterization for high resolution MALDI-MS imaging of tissue sections
Instrument design and characterization for high resolution MALDI-MS imaging of tissue sections
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DOI：
10.1002/jms.1180
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发表时间：
2007-04-01
影响因子：
2.3
通讯作者：
Caprioli, Richard M.
中科院分区：
文献类型：
--
作者：
Chaurand, Pierre;Schriver, Kenneth E.;Caprioli, Richard M.
在之前的工作中，我们报道了使用 MALDI 成像飞行时间质谱仪检测组织切片中的蛋白质离子，空间分辨率为 25 μm。我们在此展示使用高分辨率扫描 MALDI 飞行时间质谱仪获得的成像质谱结果，该质谱仪配备同轴激光照明离子源，能够实现小至 40 μ m(2)（约 7 μm 直径）的照射面积。可以在高达 27 000 的分子量范围内观察到来自这些非常小的照射体积的 MALDI 生成的分析物离子信号。从基质薄膜样品和组织切片成功生成了高分辨率成像质谱图像，扫描分辨率为 10 gm 及以下。这项工作还提供了离子信号依赖性作为各种焦点和注量参数的函数的基本特征，这些参数是扩展到亚细胞水平的组织成像所需的。版权所有 (c) 2007 John Wiley & Sons, Ltd.
In previous work, we have reported using a MALDI imaging time-of-flight mass spectrometer for the detection of protein ions from tissue sections with spatial resolution of 25 mu m. We present here imaging mass spectrometry results obtained with a high-resolution scanning MALDI time-of-flight mass spectrometer, equipped with a coaxial laser illumination ion source, capable of achieving irradiation areas as small as 40 mu m(2) (ca 7 mu m diameter). MALDI-generated analyte ion signals from these very small irradiation volumes can be observed in a molecular weight range up to 27 000. High-resolution imaging mass spectrometry images were successfully generated from matrix thin film samples and tissue sections with scanning resolutions at and below 10 gm. This work also provides fundamental characterization of the ion signal dependence as a function of various focus and fluence parameters that will be required for extension to tissue imaging at the subcellular level. Copyright (c) 2007 John Wiley & Sons, Ltd.