Large inter-individual variation in isoflavone plasma concentration limits use of isoflavone intake data for risk assessment
Large inter-individual variation in isoflavone plasma concentration limits use of isoflavone intake data for risk assessment
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异黄酮血浆浓度的巨大个体差异限制了异黄酮摄入数据用于风险评估的使用
DOI：
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发表时间：
2014
影响因子：
4.7
通讯作者：
A. Geelen
中科院分区：
文献类型：
--
作者：
V. V. D. Velpen;Peter C. H. Hollman;M. Nielen;E. Schouten;Marco Mensink;P. V. '. Veer;A. Geelen
背景/目的：异黄酮类存在于大豆食品和大豆补充剂中。尽管一般西方人群的血浆异黄酮浓度较低，但补充剂使用者的浓度超过了亚洲人群中被认为对健康有益的浓度，这引发了人们对不良反应的担忧。为了辅助风险评估，对异黄酮类摄入量和血浆浓度之间的关系进行量化是必要的。受试者/方法：来自三个安慰剂对照交叉研究的绝经后妇女的血浆样本，包括8周的补充剂(2个研究，每天100 的异黄酮量，n=88)或4周的豆制品(1个研究，~48 mg的异黄酮量/天，n=15)。血浆中异黄酮类成分(大豆苷元、木酚、染料木素和大豆黄素)采用高效液相色谱和电化学检测法进行测定。结果：豆制品和补充剂干预后血浆总异黄酮浓度中位数分别为2.16和3.47mmol.L，非中位数血浆总异黄酮浓度分别为1.30和2.39 μ μ/L。回归分析显示，增加一倍的异黄酮摄入量使血浆浓度增加55-62%(±S.E.大豆苷元、染料木素、木醇(仅适用于生产者)和总异黄酮的关联度为1-2%，R2>；0.87，而大豆黄素的关联度较弱(15±1%，R2=0.48)。对能量、碳水化合物和脂肪摄入量的调整不会影响这些估计。在其中一项研究中，基于重复测量的个体间差异估计为30%-96%。结论：尽管异黄酮类摄入量和血浆浓度之间的关系得到了充分的量化，但由于血浆浓度的个体间差异较大，使用异黄酮类摄入量数据进行风险评估需要谨慎。
Background/objectives:Isoflavones are present in soy foods and soy-based supplements. Despite low plasma isoflavone concentrations in the general Western population, concentrations in supplement users exceed those suggested to be beneficial for health in Asian populations, raising concerns for adverse effects. To aid risk assessment, quantification of the relation between isoflavone intake and plasma concentrations is essential.Subjects/methods:Plasma samples were collected from postmenopausal women in three placebo-controlled crossover studies with 8-week periods for supplements (two studies, ~100 mg isoflavones/day, n=88) or 4-week periods for soy foods (one study, ~48 mg isoflavones/day, n=15). Plasma isoflavone concentrations (daidzein, equol, genistein and glycitein) were quantified using high-performance liquid chromatography and electrochemical detection. The association between plasma concentrations and isoflavone intake, equol producer status, intake–producer interaction and background dietary intake was assessed based on the assumption of a log-linear relation.Results:Median plasma total isoflavone concentrations after the soy food and supplement interventions were respectively 2.16 and 3.47 μmol/l for equol producers and 1.30 and 2.39 μmol/l for non-producers. Regression analysis showed that doubling isoflavone intake increased plasma concentrations by 55–62% (±s.e. 1–2%, R2>0.87) for daidzein, genistein, equol (only for producers) and total isoflavones; for glycitein the association was weaker (15±1%, R2=0.48). Adjustments for energy, carbohydrate and fat intake did not affect these estimates. Inter-individual variation, estimated based on repeated measures in one of the studies, was 30–96%.Conclusions:Although the relation between isoflavone intake and plasma concentrations was adequately quantified, the use of isoflavone intake data for risk assessment needs caution due to large inter-individual variation in plasma concentrations.
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影响因子：
4.2
作者：
Setchell, KDR;Brown, NM;Heubi, JE
通讯作者：
Heubi, JE
影响因子：
45.3
作者：
Quella, SK;Loprinzi, CL;Novotny, PJ
通讯作者：
Novotny, PJ
影响因子：
4.2
作者：
Setchell, KDR;Brown, NM;Heubi, JE
通讯作者：
Heubi, JE
DOI：
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发表时间：
2010
期刊：
--
影响因子：
--
作者：
M. Messina
通讯作者：
M. Messina