Immunohistochemical localization in the rat brain of the precursor for thyrotropin-releasing hormone.
Immunohistochemical localization in the rat brain of the precursor for thyrotropin-releasing hormone.
复制标题
促甲状腺素释放激素前体在大鼠脑中的免疫组织化学定位。
DOI：
10.1126/science.3929378
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Lechan,RM
中科院分区：
文献类型：
--
作者：
Jackson,IM;Wu,P;Lechan,RM
用人工合成的十肽Cys-Lys-Arg-Gln-His-Pro-Gly-Lys-Arg-Cys(proTRH-SH)免疫制备了兔抗促甲状腺激素释放激素(ProTRH)前体多肽序列的抗血清。对大鼠脑组织的免疫组织化学研究显示，在下丘脑室旁核的小细胞部和延髓的中缝复合体中有神经元核膜的染色，与促甲状腺激素释放激素(TRH)的染色结果相同。PRTRH-SH(10~(-6)M)预先孵育可使免疫染色消失，但TRH(10~(-5)M)不能。TRH前体和TRH均定位于室旁核神经元。然而，与TRH的结果相反，在正中隆起的轴突终末没有观察到染色。这些结果表明，大鼠脑中存在一种与青蛙皮肤中报道的类似的TRH前体，哺乳动物中枢神经系统中的TRH是核糖体生物合成的产物。
A rabbit antiserum to a peptide sequence present in the precursor for thyrotropin-releasing hormone (proTRH), deduced from cloned amphibian-skin complementary DNA, was raised by immunization with the synthetic decapeptide Cys-Lys-Arg-Gln-His-Pro-Gly-Lys-Arg-Cys (proTRH-SH). Immunohistochemical studies on rat brain tissue showed staining of neuronal perikarya in the parvicellular division of the paraventricular nucleus of the hypothalamus and the raphe complex of the medulla, identical to that already described for thyrotropin-releasing hormone (TRH). Immunostaining was abolished by preincubation with proTRH-SH (10-6M) but not TRH (10-5M). Both TRH precursor and TRH were located in neurons of the paraventricular nucleus. However, in contrast to the findings for TRH, no staining was observed in axon terminals of the median eminence. These results suggest that a TRH precursor analogous to that reported in frog skin is present in the rat brain and that TRH in the mammalian central nervous system is a product of ribosomal biosynthesis.