A serotonin neurotoxin attenuates the phase-shifting effects of triazolam on the circadian clock in hamsters.
A serotonin neurotoxin attenuates the phase-shifting effects of triazolam on the circadian clock in hamsters.
复制标题
血清素神经毒素会减弱三唑仑对仓鼠生物钟的相移作用。
DOI：
10.1016/0006-8993(94)01237-c
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发表时间：
1995
期刊：
影响因子：
2.9
通讯作者：
Zee,PC
中科院分区：
文献类型：
--
作者：
Penev,PD;Turek,FW;Zee,PC
一些证据表明，在介导活动诱导刺激对啮齿动物的生物钟的影响中，多巴胺能通路可能参与其中。本实验的目的是研究肾上腺素能神经毒素对氯苯丙胺的作用（PCA，10 mg/kg）：（1）仓鼠下丘脑、额叶皮层和海马的单胺水平;（2）仓鼠对短效苯二氮卓类药物、三唑仑治疗的反应的运动活动昼夜节律的相移（7.5 mg/kg）;和（3）在该物种中与三唑仑给药相关的自发活动急性增加的幅度。给仓鼠施用五氯苯甲醚引起下丘脑中特定单胺能系统的变化，仅限于治疗后7天血清素水平的选择性降低。在CT 6处，响应于三唑仑治疗的生物钟的相移在5-HT神经毒素给药后显著减弱。PCA处理对CT 6和CT 12之间三唑仑诱导的轮跑和一般笼活动的总量和曲线没有显著影响。5-HT神经毒素可以减弱三唑仑在仓鼠中的相移效应，而不干扰其活动诱导特性，这一发现表明，多巴胺能传入可能参与昼夜节律系统的非光相移机制。
Several lines of evidence suggest the potential involvement of serotonergic pathways in mediating the effects of activity-inducing stimuli on the circadian clock in rodents. The aim of the present 3 experiments was to examine the effects of the serotonergic neurotoxin, p-hloroamphetamine (PCA, 10 mg/kg) on: (1) the monoamine levels of the hypothalamus, frontal cortex and hippocampus in the hamster; (2) the phase shifts in the circadian rhythm of locomotor activity of hamsters in response to treatment with the short-acting benzodiazepine, triazolam (7.5 mg/kg); and (3) the magnitude of the acute increase in locomotor activity associated with triazolam administration in this species. The administration of PCA to hamsters caused changes of specific monoaminergic systems in the hypothalamus, that were limited to a selective decrease in serotonin levels 7 days post-treatment. The phase shifts of the circadian clock in response to triazolam treatment at CT 6 were considerably attenuated following the administration of the 5-HT neurotoxin. The total amount and the profiles of triazolam-induced wheel-running and general cage activity between CT 6 and CT 12 were not significantly affected by the PCA treatment. The finding that a 5-HT neurotoxin can attenuate the phase-shifting effects of triazolam in hamsters, without interfering with its activity-inducing properties, suggests that serotonergic afferents might be involved in the mechanism for non-photic phase-shifting of the circadian system.