Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries
Optical-resolution photoacoustic microscopy for in vivo imaging of single capillaries
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DOI：
10.1364/ol.33.000929
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发表时间：
2008-05-01
期刊：
影响因子：
3.6
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Maslov, Konstantin;Zhang, Hao F.;Wang, Lihong V.
毛细血管是最小的血管，是脉管系统的远端，血液和组织之间交换氧气和营养物质。因此，长期以来，人们一直希望将毛细血管和体内功能的无创成像作为研究神经血管耦合等基础生理学的窗口。现有的成像方式无法提供所需的灵敏度和空间分辨率。我们使用我们实验室开发的光学分辨率光声显微镜（OR-PAM）对小鼠的微脉管系统（包括单个毛细血管）进行体内成像。 OR-PAM 提供 5 μm 的横向分辨率和 >0.7 mm 的成像深度。 (C) 2008 年美国光学学会。
Capillaries, the smallest blood vessels, are the distal end of the vasculature where oxygen and nutrients are exchanged between blood and tissue. Hence, noninvasive imaging of capillaries and function in vivo has long been desired as a window to studying fundamental physiology, such as neurovascular coupling. Existing imaging modalities cannot provide the required sensitivity and spatial resolution. We present in vivo imaging of the microvasculature including single capillaries in mice using optical-resolution photoacoustic microscopy (OR-PAM) developed in our laboratory. OR-PAM provides a lateral resolution of 5 mu m and an imaging depth >0.7 mm. (C) 2008 Optical Society of America.