SOX2-positive retinal stem cells are identified in adult human pars plicata by single-cell transcriptomic analyses.
SOX2-positive retinal stem cells are identified in adult human pars plicata by single-cell transcriptomic analyses.
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作者：
干细胞治疗是一种很有前途的策略，以挽救视网膜变性引起的视力障碍。先前的研究提出了关于成人眼组织中是否存在原位视网膜干细胞（RSCs）的有争议的理论。单细胞RNA测序（scRNA-seq）已成为揭示组织细胞异质性的最有力工具之一。通过使用scRNA-seq，我们探索了成年人眼睛不同亚区的细胞异质性，包括睫状体、睫状体平坦部、视网膜色素上皮（RPE）、虹膜和神经视网膜（NR）。我们鉴定了一个表达SRY盒转录因子2（SOX 2）的亚群作为RSC，其存在于成年人眼睛的睫状体中。进一步的分析表明，鉴定的RSC亚群表达特异性标志物水通道蛋白1（AQP 1）和四跨膜蛋白12（TSPAN 12）。因此，我们使用这两种标记物通过流式分选分离出这个亚群，发现分离的RSC可以增殖并分化为一些视网膜细胞类型，包括光感受器、神经元、RPE细胞、小胶质细胞、星形胶质细胞、水平细胞、双极细胞和神经节细胞;而AQP 1 − TSPAN 12 −细胞不具有这种分化潜力。总之，我们的研究结果表明，SOX 2阳性RSC存在于睫状体中，由于其增殖和分化潜力，可能对治疗人类视网膜疾病有价值。SOX 2阳性视网膜干细胞（RSC）在成人睫状体中被鉴定。以水通道蛋白1和四跨膜蛋白12为特异性表面标志物，分离培养大鼠肾干细胞，证明其具有增殖和分化能力。
Stem cell therapy is a promising strategy to rescue visual impairment caused by retinal degeneration. Previous studies have proposed controversial theories about whether in situ retinal stem cells (RSCs) are present in adult human eye tissue. Single‐cell RNA sequencing (scRNA‐seq) has emerged as one of the most powerful tools to reveal the heterogeneity of tissue cells. By using scRNA‐seq, we explored the cell heterogeneity of different subregions of adult human eyes, including pars plicata, pars plana, retinal pigment epithelium (RPE), iris, and neural retina (NR). We identified one subpopulation expressing SRY‐box transcription factor 2 (SOX2) as RSCs, which were present in the pars plicata of the adult human eye. Further analysis showed the identified subpopulation of RSCs expressed specific markers aquaporin 1 (AQP1) and tetraspanin 12 (TSPAN12). We, therefore, isolated this subpopulation using these two markers by flow sorting and found that the isolated RSCs could proliferate and differentiate into some retinal cell types, including photoreceptors, neurons, RPE cells, microglia, astrocytes, horizontal cells, bipolar cells, and ganglion cells; whereas, AQP1− TSPAN12− cells did not have this differentiation potential. In conclusion, our results showed that SOX2‐positive RSCs are present in the pars plicata and may be valuable for treating human retinal diseases due to their proliferation and differentiation potential. SOX2‐positive retinal stem cells (RSCs) are identified in adult human pars plicata. Using the specific surface markers aquaporin 1 and tetraspanin 12, the RSCs were isolated and proved to have proliferation and differentiation abilities.
影响因子：
5.2
作者：
Inoue, Tomoyuki;Coles, Brenda L. K.;Dorval, Kim;Bremner, Rod;Bessho, Yasumasa;Kageyama, Ryoichiro;Hino, Shinjiro;Matsuoka, Masao;Craft, Cheryl M.;McInnes, Roderick R.;Tremblay, Francois;Prusky, Glen T.;van der Kooy, Derek
通讯作者：
van der Kooy, Derek