Differential expression of apoptosis-related Fas antigen on lymphocyte subpopulations in human peripheral blood.
Differential expression of apoptosis-related Fas antigen on lymphocyte subpopulations in human peripheral blood.
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人外周血淋巴细胞亚群凋亡相关Fas抗原的差异表达。
DOI：
10.4049/jimmunol.149.11.3753
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发表时间：
1992
影响因子：
4.4
通讯作者：
N. Taniguchi
中科院分区：
文献类型：
--
作者：
T. Miyawaki;T. Uehara;R. Nibu;T. Tsuji;A. Yachie;S. Yonehara;N. Taniguchi
Fas Ag 是一种新定义的细胞表面分子，可能介导细胞凋亡。针对 Fas Ag 的抗体可以诱导表达该 Ag 的细胞系中的细胞凋亡。这里使用抗 Fas mAb 通过二色或三色流式细胞术分析检查不同年龄的 PBL 亚群的 Fas 表达。研究发现，部分 T 细胞和 B 细胞可明显检测到 Fas Ag，而 NK 细胞则不表达 Fas Ag。对于 CD4+ 和 CD8+ T 细胞，Fas Ag 优先在 CD45RO+（记忆或先前激活的）细胞群上表达，但不在 CD45RO- 初始细胞群上表达。 TCR-gamma/delta+ T 细胞，尤其是其 CD45RO+ 亚群，也表达 Fas Ag。不出所料，新生儿 T 细胞亚群（其中大多数具有幼稚（CD45RO-）表型）表达很少的 Fas Ag。表达 Fas 的 B 细胞在表面 IgD- 群体中占主导地位，但新生儿 B 细胞以及成人 sIgD+ B 细胞几乎没有 Fas Ag。 Fas Ag 在体外对新生儿血液中的幼稚 T 和 B 细胞进行有丝分裂刺激后可诱导。这些观察结果表明，外周血中 T 细胞和 B 细胞上 Fas Ag 的表达可能反映了它们体内 Ag 激活的状态。然而，与表达 Fas 的培养细胞系相比，体外刺激的 T 细胞和 B 细胞以及新鲜分离的 CD45RO+ T 细胞的活力在用抗 Fas mAb 处理后没有显着变化，表明抗 Fas 诱导的细胞死亡可能需要额外的细胞条件来表达 Fas。
The Fas Ag is a newly defined cell-surface molecule that may mediate apoptosis. The antibody against Fas Ag can induce the apoptotic cell death in cell lines expressing this Ag. PBL subpopulations at various ages were here examined for Fas expression by two-or three-color flow-cytometric analyses using anti-Fas mAb. It was found that Fas Ag was appreciably detected on a proportion of T and B cells, whereas its expression was absent for NK cells. For CD4+ and CD8+ T cells, Fas Ag was expressed preferentially on CD45RO+ (memory or previously activated) populations, but not on CD45RO- naive ones. TCR-gamma/delta+ T cells, especially their CD45RO+ subsets, also expressed Fas Ag. Expectably, neonatal T cell subpopulations, most of which had the naive (CD45RO-) phenotype, expressed little Fas Ag. Fas-expressing B cells dominated in surface(s) IgD- populations, but neonatal B cells as well as adult sIgD+ B cells had little Fas Ag. The Fas Ag was inducible after in vitro mitogenic stimulation of naive T and B cells from neonatal blood. These observations suggested that expression of Fas Ag on T and B cells in the peripheral blood might reflect their in vivo Ag-activated status. In contrast to Fas-expressing cultured cell lines, however, viability of in vitro stimulated T and B cells as well as freshly isolated CD45RO+ T cells was not significantly changed after the treatment with anti-Fas mAb, indicating that additional cellular conditions to Fas expression might be required for anti-Fas-induced cell death.