Collagenase-3 (MMP-13) is expressed in periapical lesions: an immunohistochemical study
Collagenase-3 (MMP-13) is expressed in periapical lesions: an immunohistochemical study
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DOI：
10.1111/j.1365-2591.2005.00943.x
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发表时间：
2005-05-01
影响因子：
5
通讯作者：
Loreto, C
中科院分区：
文献类型：
--
作者：
Leonardi, R;Caltabiano, R;Loreto, C
目的探讨基质金属蛋白酶13（matrix metalloproteinase 13，MMP-13）在牙髓根尖周肉芽肿中的表达及其与牙髓上皮的关系。方法将17例牙髓根尖周肉芽肿（7例无上皮，10例有增生上皮）用福尔马林固定，石蜡包埋，进行免疫组化分析。使用抗人MMP-13的单克隆抗体来评估MMP-13表达。随后用二抗处理免疫复合物，然后通过链霉亲和素过氧化物酶检测。结果MMP-13在所有标本中均呈胞浆免疫阳性反应，但抗MMP-13抗体的免疫染色是异质性的，其水平根据组织病理学结果而变化。在根尖周病变无上皮MMP-13免疫标记检测到一些成纤维细胞样细胞，并在一些浆细胞内的肉芽肿组织。MMP-13在根尖周病变中的表达明显上调，尤其是在小的上皮岛和细股上皮中。结论MMP-13的表达模式表明，它参与了根尖周肉芽肿上皮向根尖囊肿的转化。这种特性与MMP-13不仅影响上皮细胞的迁移而且影响肉芽肿组织的侵袭的能力有关。
Aim To determine whether or not matrix metalloproteinase 13 (MMP-13) is present in periapical granulomas with and without epithelium.Methodology Seventeen open periapical granulomas of pulpal origin (seven lesions without epithelium and 10 with proliferating epithelium) were fixed in formalin and then embedded in paraffin prior to being processed for immunohistochemical analysis. A monoclonal antibody against human MMP-13 was used to evaluate MMP-13 expression. Immunocomplexes were subsequently treated with the secondary antibody and then detected by means of streptavidin peroxidase. Immunoreactivity was visualized by development with 3,3'-diaminobenzidine.Results An immunopositive cytoplasmatic reaction for MMP-13 was observed in all the specimens, although the immunostaining by anti-MMP-13 antibody was heterogeneous and its levels varied according to histopathological findings. In periapical lesions without epithelium MMP-13 immunolabelling was detected in a few fibroblast-like cells, and in some plasma cells within the granulomatous tissue. A clear upregulation of MMP-13 expression was detected in periapical lesions with epithelium, especially in small island and thin strands of epithelium.Conclusions The expression pattern of MMP-13 demonstrates that it is involved in the conversion of a periapical granuloma with epithelium into a radicular cyst. This property is related to the ability of MMP-13 to influence not only the migration of epithelial cell but also the invasion of granulomatous tissue.