Decreased serum estrogen improves fat graft retention by enhancing early macrophage infiltration and inducing adipocyte hypertrophy
Decreased serum estrogen improves fat graft retention by enhancing early macrophage infiltration and inducing adipocyte hypertrophy
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血清雌激素减少通过增强早期巨噬细胞浸润和诱导脂肪细胞肥大来改善脂肪移植保留
DOI：
10.1016/j.bbrc.2018.04.232
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发表时间：
2018-06-18
影响因子：
3.1
通讯作者：
Lu, Feng
中科院分区：
文献类型：
--
作者：
Mok, Hsiaopei;Feng, Jingwei;Lu, Feng
背景：脂肪移植是一种常用的手术，然而，调节移植结果的机制尚不清楚。雌激素与血管形成、炎症和脂肪代谢有关，但其在脂肪移植中的作用尚不清楚。方法：小鼠植入17 β-雌二醇颗粒（高雌激素，HE），进行卵巢切除术（低雌激素水平，OVX）或假手术（正常雌激素水平，CON）。45天后，将小鼠腹股沟脂肪皮下自体移植。在移植后1，2，4，12周，移植物被解剖，称重，并评估组织学，血管生成和炎症level.Results：血清雌激素水平与雌激素的操作。自体移植12周后，OVX组的留存率（79% ± 30%）显著高于HE组（16% ± 8%）和CON组（35% ± 13%）。OVX移植物有最少的坏死和最肥大的脂肪。OVX招募了最促炎的巨噬细胞，并显示出更快的死亡组织清除过程，然而，在该组中发现了更高的纤维化倾向。HE移植物具有最多的Scal+局部干细胞和CD31（+）毛细血管内容物;然而，急性炎症水平较低，脂肪因子PPAR-gamma表达不足，其留存率受损。结论：血清雌激素升高可增加干细胞密度和早期血管化;然而，通过抑制早期炎症，它导致延迟坏死组织清除，最终导致脂肪恢复受损。低雌激素水平诱导良好的炎症状态和脂肪细胞肥大，以改善脂肪移植物的保留，但持续降低雌激素水平导致脂肪移植物纤维化。(C)2018爱思唯尔公司All rights reserved.
Background: Fat grafting is a commonly used procedure; however, the mechanisms that regulate graft outcomes are not clear. Estrogen has been associated with vascularization, inflammation and fat metabolism, yet its role in fat grafting is unclear.Methods: Mice were implanted with 17 beta-estradiol pellets (high estrogen, HE), underwent ovariectomy (low estrogen level, OVX) or sham surgery (normal estrogen level, CON). 45 days later, inguinal fat of mice was autografted subcutaneously. At 1, 2, 4, and 12 weeks post-transplantation, grafts were dissected, weighed, and assessed for histology, angiogenesis and inflammation level.Results: Serum estrogen level correlated to estrogen manipulation. 12 weeks after autografting, the retention rate was significantly higher in the OVX (79% +/- 30%) than in the HE (16% +/- 8%) and CON (35% +/- 13%) groups. OVX-grafts had the least necrosis and most hypertrophic fat. OVX recruited the most pro-inflammatory macrophages and demonstrated a faster dead tissue removal process, however a higher fibrogenic tendency was found in this group. HE grafts had the most Scal + local stem cells and CD31(+) capillary content; however, with a low level of acute inflammation and insufficient adipokine PPAR-gamma expression, their retention rate was impaired.Conclusions: Elevated serum estrogen increased stem cell density and early vascularization; however, by inhibiting the early inflammation, it resulted in delayed necrotic tissue removal and finally led to impaired adipose restoration. A low estrogen level induced favorable inflammation status and adipocyte hypertrophy to improve fat graft retention, but a continuing decreased estrogen level led to fat graft fibrosis. (C) 2018 Elsevier Inc. All rights reserved.