Extract of Oregano, Coffee, Thyme, Clove, and Walnuts Inhibits NF-κB in Monocytes and in Transgenic Reporter Mice
Extract of Oregano, Coffee, Thyme, Clove, and Walnuts Inhibits NF-κB in Monocytes and in Transgenic Reporter Mice
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DOI：
10.1158/1940-6207.capr-09-0089
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发表时间：
2010-05-01
影响因子：
3.3
通讯作者：
Blomhoff, Rune
中科院分区：
文献类型：
--
作者：
Paur, Ingvild;Balstad, Trude R.;Blomhoff, Rune
转录因子 NF-kappa B 是化学预防的一个有前景的靶标。几种膳食植物在体外是 NF-kappa B 激活的有效抑制剂，并且可以对 NF-kappa B 信号通路产生协同作用。在本研究中，我们测试了膳食植物提取物是否可以在体外以协同方式抑制 NF-κ B 激活。其次，我们研究了相同膳食植物提取物在体内抑制 NF-κ B 激活的效力。与单一提取物的效果总和相比，丁香、牛至、百里香、核桃和咖啡的组合提取物可协同抑制单核细胞系中脂多糖 (LPS) 诱导的 NF-κ B 激活。转基因 NF-κ B 荧光素酶报告小鼠接受单剂量的组合提取物，随后用 LPS 进行攻击。通过体内成像监测 NF-κ B 激活 6 小时。此外，还研究了器官中的NF-κB活性和肝脏中免疫相关基因的表达。根据曲线下面积，与对照小鼠相比，提取物在前 6 小时内将 LPS 诱导的全身 NF-κ B 活性降低了 35%。接受联合提取物的小鼠的肠、肝脏、睾丸和附睾中器官特异性 NF-κ B 激活受到抑制。此外，膳食植物还减少了与肝脏炎症、细胞迁移和增殖相关的基因表达。这项研究表明，膳食植物可能是体外和体内 NF-κ B 信号传导的有效调节剂，因此支持进一步研究将这些植物性食品作为健康饮食的一部分或作为化学预防的一种模式。癌症预防研究； 3（5）； 653-63。 (C) 2010 AACR。
The transcription factor NF-kappa B is a promising target for chemoprevention. Several dietary plants are efficient inhibitors of NF-kappa B activation in vitro and could act synergistically on the NF-kappa B signaling pathway. In this study, we tested whether dietary plant extracts could inhibit NF-kappa B activation in a synergistic manner in vitro. Second, we investigated the potency of the same dietary plant extracts in the inhibition of NF-kappa B activation in vivo. A combined extract of clove, oregano, thyme, walnuts, and coffee synergistically inhibited lipopolysaccaride (LPS)-induced NF-kappa B activation in a monocytic cell line, compared with the sum of effects from the single extracts. Transgenic NF-kappa B luciferase reporter mice were given a single dose of the combined extract and subsequently challenged with LPS. NF-kappa B activation was monitored by in vivo imaging for 6 hours. In addition, NF-kappa B activity in organs and the expression of immune-related genes in liver were investigated. Based on the area under the curve, the extract decreased whole body LPS-induced NF-kappa B activity the first 6 hours by 35% compared with control mice. Organ-specific NF-kappa B activation was inhibited in intestine, liver, testis, and epididymis of the mice receiving the combination extract. In addition, dietary plants reduced the expression of genes related to inflammation, cell migration, and proliferation in liver. This study shows that dietary plants may be potent modulators of NF-kappa B signaling both in vitro and in vivo, and thus support further investigation of consumption of these plant foods as part of a healthy diet or as a mode of chemoprevention. Cancer Prev Res; 3(5); 653-63. (C) 2010 AACR.