Up-regulation of Cav3.1 expression in SH-SY5Y cells induced by lidocaine hydrochloride
Up-regulation of Cav3.1 expression in SH-SY5Y cells induced by lidocaine hydrochloride
复制标题
盐酸利多卡因诱导SH-SY5Y细胞Cav3.1表达上调
DOI：
10.1080/21691401.2018.1425697
复制
发表时间：
2018-01-01
影响因子：
5.8
通讯作者：
Wang, Xiaoping
中科院分区：
文献类型：
--
作者：
Gong, Qin;Wen, Xianjie;Wang, Xiaoping
摘要背景局麻药的神经毒性已引起人们的关注。先前的研究表明，细胞内钙超载参与神经毒性作用。Cav3.1是一种低电压激活的钙通道，在调节细胞内钙离子水平中起关键作用。本研究旨在探讨盐酸利多卡因对SH-SY 5 Y细胞Cav 3. 1表达的影响。方法采用不同浓度盐酸利多卡因（1 mM、5 mM和10 mM，即L1组、L5组和L10组）和不同作用时间（1 h、12 h和24 h）处理SH-SY 5 Y细胞。检测细胞活力、Cav3.1蛋白和mRNA表达。结果随着盐酸利多卡因浓度（1 ~ 10 mM）和作用时间（1 ~ 24 h）的增加，细胞存活率降低，Cav 3. 1 mRNA和蛋白表达增加。结论盐酸利多卡因诱导SH-SY 5 Y细胞毒性，上调Cav 3. 1 mRNA和蛋白表达。
Abstract Background Neurotoxicity induced by the local anaesthetics has aroused concern. A previous study has shown that an overload of intracellular calcium was involved in the neurotoxic effect. Cav3.1 is one of the low-voltage-activated (LVA) calcium channels which play a key point to regulate the intracellular calcium ion level. This study aimed to investigate the changes of the Cav3.1 expression in the SH-SY5Y cells treated with lidocaine hydrochloride. Methods The SH-SY5Y cells were treated with different concentrations of lidocaine hydrochloride(1 mM, 5 mM and 10 mM, namely L1 group, L5 group and L10 group) and different exposure times (1 h,12 h and 24 h), respectively. Cell viability, Cav3.1 protein and mRNA expression were detected. Results The results showed that cell viability decreased and Cav3.1 mRNA and protein expression increased with the concentration (from 1 mM to 10 mM) of the lidocaine hydrochloride and exposure time (from 1 h to 24 h) to the SH-SY5Y cell line increased. Conclusion Those data showed that lidocaine hydrochloride induced SH-SY5Y cell toxicity and up-regulated Cav3.1mRNA and protein expression.