Multiple (x and /? Tubulin Genes Represent Unlinked and Dispersed Gene Families*
Multiple (x and /? Tubulin Genes Represent Unlinked and Dispersed Gene Families*
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多个 (x 和 /? 微管蛋白基因代表未连锁和分散的基因家族*
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
Varmus
中科院分区：
文献类型：
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作者：
Don.;W.;ClevelandSg;Stephen;H.;Hughes;E. Stubblefield;Marc;W.;KirschnerS;Harold;E.;Varmus
克隆的特异于α或β微管蛋白mRNA的cDNA序列已被用于表明编码α或β微管蛋白的多基因家族是不连锁的，并分散在整个鸡基因组中。用蔗糖梯度离心法部分纯化鸡染色体，用限制性内切酶酶切，阿加糖凝胶电泳。将DNA转移到硝酸纤维素滤膜上，并与标记探针杂交，所述标记探针由特异于α或β微管蛋白的克隆cDNA序列构建。我们发现一个微管蛋白序列在四个不同的鸡染色体和/3微管蛋白序列在至少两个不同的染色体上。此外，使用用荧光细胞分选仪进一步纯化的鸡染色体，我们已经能够明确地将微管蛋白基因定位于1号染色体和8号染色体，并将2个/3基因定位于2号染色体。
Cloned cDNA sequences specific for a or /3 tubulin mRNAs have been used to show that the multigene families which encode either a or /3 tubulin are unlinked and dispersed throughout the chicken genome. Fractions of chicken chromosomes partially purified by cen- trifugation on a sucrose gradient were digested with restriction endonucleases and electrophoresed on aga- rose gels. The DNA was transferred to nitrocellulose filters and hybridized to labeled probes constructed from cloned cDNA sequences specific for a or /3 tubulin. We find a tubulin sequences on four different chicken chromosomes and /3 tubulin sequences on at least two different chromosomes. Moreover, using chicken chro- mosomes further purified with a fluorescent cell sorter, we have been able unambiguously to localize a tubulin genes to chromosome 1 and chromosome 8 and two of the /3 genes to chromosome 2.