A Satellite dsRNA Attenuates the Induction of Helper Virus-Mediated Symptoms in Aspergillus flavus.
A Satellite dsRNA Attenuates the Induction of Helper Virus-Mediated Symptoms in Aspergillus flavus.
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DOI：
10.3389/fmicb.2022.895844
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发表时间：
2022
影响因子：
5.2
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中科院分区：
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黄曲霉是一种重要的动植物病原真菌。以前，我们报道了一种新的分离病毒，黄曲霉分离病毒1（AfPV1），感染A。黄的在这项研究中，我们获得了一个小的双链（ds）RNA片段（734 bp），这是一个卫星RNA的辅助病毒，AfPV1。AfPV1的存在改变了殖民地形态，减少了分生孢子梗的数量，产生了明显更大的液泡，并导致A.而小RNA片段能减轻辅助病毒AfPV1引起的上述症状。黄的AfPV1感染降低了A.玉米（Zea mays）、蜂窝蛾（Galleria mellonella）、小鼠（Mus musculus）中的黄曲霉，以及分生孢子对宿主上皮细胞的粘附，以及增加巨噬细胞引起的分生孢子死亡。然而，小RNA片段也可以减轻辅助病毒AfPV1在A.这可能是通过减少辅助病毒AfPV1在A.黄的我们利用该模型研究了由AfPV1和A. flavus，以及它们在产生不同表型中的作用。我们发现AfPV1在其宿主中调控的基因途径与逆转录病毒相似。因此，一些途径可能有利于非逆转录病毒整合或内源化到其宿主的基因组中。此外，在A. Flavus使用这个系统。
Aspergillus flavus is an important fungal pathogen of animals and plants. Previously, we reported a novel partitivirus, Aspergillus flavus partitivirus 1 (AfPV1), infecting A. flavus. In this study, we obtained a small double-stranded (ds) RNA segment (734 bp), which is a satellite RNA of the helper virus, AfPV1. The presence of AfPV1 altered the colony morphology, decreased the number of conidiophores, created significantly larger vacuoles, and caused more sensitivity to osmotic, oxidative, and UV stresses in A. flavus, but the small RNA segment could attenuate the above symptoms caused by the helper virus AfPV1 in A. flavus. Moreover, AfPV1 infection reduced the pathogenicity of A. flavus in corn (Zea mays), honeycomb moth (Galleria mellonella), mice (Mus musculus), and the adhesion of conidia to host epithelial cells, and increased conidial death by macrophages. However, the small RNA segment could also attenuate the above symptoms caused by the helper virus AfPV1 in A. flavus, perhaps by reducing the genomic accumulation of the helper virus AfPV1 in A. flavus. We used this model to investigate transcriptional genes regulated by AfPV1 and the small RNA segment in A. flavus, and their role in generating different phenotypes. We found that the pathways of the genes regulated by AfPV1 in its host were similar to those of retroviral viruses. Therefore, some pathways may be of benefit to non-retroviral viral integration or endogenization into the genomes of its host. Moreover, some potential antiviral substances were also found in A. flavus using this system.