Effects of high-intensity training and acute exercise on in vitro function of rat sarcoplasmic reticulum
Effects of high-intensity training and acute exercise on in vitro function of rat sarcoplasmic reticulum
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DOI：
10.1007/s00421-006-0381-8
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发表时间：
2007-01
影响因子：
3
通讯作者：
S. Matsunaga;Takashi Yamada;T. Mishima;M. Sakamoto;Minako Sugiyama;M. Wada
中科院分区：
文献类型：
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作者：
S. Matsunaga;Takashi Yamada;T. Mishima;M. Sakamoto;Minako Sugiyama;M. Wada
为评价大强度训练和(或)单次运动对肌浆网(SR)体外功能的影响，对大鼠进行8周的间歇跑训练(末次训练：2.5min/d×4s，50m/min，10%坡度)。训练结束后，对单次跑台跑(50m/min，10%倾斜)2.5min或力竭状态下大鼠股外侧肌浅层匀浆中的肌浆网功能，即Ca~(2+)-ATPase活性和Ca~(2+)摄取与释放速率进行了检测。训练使静息肌肉肌浆网钙摄取率提高12.4%(P<；P<；0.05)。这种变化没有伴随着钙离子-ATPase活性、钙释放速率、酶的钙依赖性以及钙离子-ATPase和兰尼定受体蛋白含量的变化。无论动物是否接受过训练，一次高强度的运动到筋疲力尽都会引起SR功能的显著降低(P<；P<；005)。对于2.5min跑和力竭运动的大鼠，未训练肌肉和训练肌肉的SR功能没有差异。这些数据表明，大强度训练可能能够提高SR的钙离子储存能力，但可能不能防止大强度运动导致SR功能的下降。
To evaluate the effects of high-intensity training and/or a single bout of exercise on in vitro function of the sarcoplasmic reticulum (SR), the rats were subjected to 8 weeks of interval running program (final training: 2.5-min running × 4 sets per day, 50 m/min at 10% incline). Following training, SR function, i.e., Ca2+-ATPase activity and Ca2+-uptake and release rates, was examined in homogenates of the superficial region of the vastus lateralis muscle from rats subjected to a single bout of treadmill running (50 m/min at 10% incline) for 2.5 min or to exhaustion. Training brought about a 12.4% increase (P< 0.05) in SR Ca2+-uptake rate in rested muscles. This change was not accompanied by alterations in Ca2+-ATPase activity, Ca2+-release rate, Ca2+dependence of enzyme and protein contents of Ca2+-ATPase and ryanodine receptor. A single bout of high-intensity exercise to exhaustion evoked significant reductions (P< 0.05) in SR function, irrespective of whether or not the animals were trained. For 2.5-min run and exhausted rats, no differences existed between SR functions of untrained and trained muscles. These data suggest that high-intensity training may be capable of enhancing SR Ca2+-sequestering ability, and may not protect against decreasing SR function with high-intensity exercise.