Identification of a third region of cell-specific alternative splicing in human fibronectin mRNA.
Identification of a third region of cell-specific alternative splicing in human fibronectin mRNA.
复制标题
鉴定人纤连蛋白 mRNA 中细胞特异性选择性剪接的第三个区域。
DOI：
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发表时间：
1987
影响因子：
11.1
通讯作者：
A. Kornblihtt
中科院分区：
文献类型：
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作者：
Alejandro Gutman;A. Kornblihtt
我们在这里描述了第三个区域的可变性，在人纤连蛋白（FN）由于选择性RNA剪接。之前已经报道了选择性剪接的另外两个位置（艾德和IIICS）。第三个区域涉及一个273个核苷酸的外显子，编码正好一个III型同源性的91个氨基酸重复序列，位于FN的DNA和细胞结合结构域之间，该结构域包括在FN mRNA中或从FN mRNA中排除。由外显子跳跃机制产生的两种mRNA变体存在于已知合成细胞形式的FN的细胞中。然而，作为血浆FN来源的肝细胞仅产生信使，而不产生额外的III型序列。因此，这里描述的区域在结构和功能上都类似于先前描述的艾德（额外结构域）区域，位于分子的C末端，在细胞和肝素（hep 2）结合结构域之间。我们的结论是，额外的III型重复（命名为EDII）和艾德代表的序列仅限于细胞FN。迄今为止描述的三个区域中所有可能的剪接模式的组合可以从单个基因产生多达20种不同的FN多肽。
We describe here a third region of variability in human fibronectin (FN) due to alternative RNA splicing. Two other positions of alternative splicing have been reported previously (ED and IIICS). The third region involves a 273-nucleotide exon encoding exactly one 91-amino acid repeat of type III homology, located between the DNA- and the cell-binding domains of FN, which is either included in or excluded from FN mRNA. The two mRNA variants arising by an exon-skipping mechanism are present in cells known to synthesize the cellular form of FN. However, liver cells, which are the source of plasma FN, produce only messengers without the extra type III sequence. Therefore, the region described here resembles, both structurally and functionally, the previously described ED (for extra domain) region, located toward the C terminus of the molecule, between the cell- and heparin- (hep 2) binding domains. We conclude that both the extra type III repeat (named EDII) and ED represent sequences restricted to cellular FN. Combination of all the possible patterns of splicing in the three regions described to date may generate up to 20 distinct FN polypeptides from a single gene.