Talin B regulates collective cell migration via PI3K signaling in Dictyostelium discoideum mounds
Talin B regulates collective cell migration via PI3K signaling in Dictyostelium discoideum mounds
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Talin B 通过 PI3K 信号传导调节盘基网柄菌丘中的集体细胞迁移
DOI：
10.1016/j.bbrc.2020.02.060
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发表时间：
2020
影响因子：
3.1
通讯作者：
Masahiro Ueda
中科院分区：
文献类型：
--
作者：
Shin-Ichi Yamazaki;Hidenori Hashimura;Yusuke V. Morimoto;Yukihiro Miyanaga;Satomi Matsuoka;Yoichiro Kamimura;Masahiro Ueda
集体细胞迁移是多细胞生物发育过程中的一个关键过程，其中单个细胞的迁移通过细胞之间的化学引导和物理接触来协调。塔林蛋白与肌动蛋白为基础的运动机制和粘附分子之间的机械联系有关，但塔林蛋白如何促进集体细胞迁移尚不清楚。我们发现talin B在盘基网柄藻发育过程中的多细胞丘阶段参与了细胞间的化学协调，以实现细胞的集体迁移。从早期聚集到丘状体形成，talB-null细胞表现出正常的集体迁移与cAMP中继。随后，talB-null细胞在丘阶段表现出发育停滞，同时，它们已经损害了集体迁移和cAMP中继，而野生型细胞在丘阶段期间与cAMP中继一致地持续表现出旋转细胞迁移。PI 3 K活性的遗传抑制部分恢复了集体细胞迁移和cAMP中继中的talB无效表型。总之，我们的观察结果表明，talin B调节化学协调通过PI 3 K介导的信号在特定阶段的方式为多细胞发育dictyostelium细胞。
Collective cell migration is a key process during the development of multicellular organisms, in which the migrations of individual cells are coordinated through chemical guidance and physical contact between cells. Talin has been implicated in mechanical linkage between actin-based motile machinery and adhesion molecules, but how talin contributes to collective cell migration is unclear. Here we show that talin B is involved in chemical coordination between cells for collective cell migration at the multicellular mound stage in the development ofDictyostelium discoideum. From early aggregation to the mound formation,talB-null cells exhibited collective migration normally with cAMP relay. Subsequently,talB-null cells showed developmental arrest at the mound stage, and at the same time, they had impaired collective migration and cAMP relay, while wild-type cells exhibited rotational cell migration continuously in concert with cAMP relay during the mound stage. Genetic suppression of PI3K activity partially restoredtalB-null phenotypes in collective cell migration and cAMP relay. Overall, our observations suggest that talin B regulates chemical coordination via PI3K-mediated signaling in a stage-specific manner for the multicellular development ofDictyosteliumcells.