The sympathetic nervous system is controlled by transient receptor potential vanilloid 1 in the regulation of body temperature.
The sympathetic nervous system is controlled by transient receptor potential vanilloid 1 in the regulation of body temperature.
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DOI：
10.1096/fj.15-272526
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发表时间：
2015-10
期刊：
影响因子：
--
通讯作者：
Keeble JE
中科院分区：
文献类型：
--
作者：
Alawi KM;Aubdool AA;Liang L;Wilde E;Vepa A;Psefteli MP;Brain SD;Keeble JE
瞬时受体电位香草酸 1 (TRPV1) 参与感觉神经伤害性信号传导。最近，人们发现 TRPV1 受体还可以调节从小鼠到人类的多个物种的基础体温。在本研究中，我们研究了 TRPV1 是否调节基础交感神经系统 (SNS) 活动。 C57BL6/J野生型（WT）小鼠和TRPV1敲除（KO）小鼠被植入无线电遥测探针以测量核心体温。腹膜内注射AMG9810 (50 mg/kg)或媒介物(2% DMSO/5%吐温80/10 ml/kg盐水)。皮下注射肾上腺素受体拮抗剂或媒介物(5ml/kg盐水)。在 WT 小鼠中，TRPV1 拮抗剂 AMG9810 引起明显的体温过高，与棕色脂肪组织中去甲肾上腺素浓度增加有关。 β-肾上腺素受体拮抗剂普萘洛尔、混合α-/β-肾上腺素受体拮抗剂拉贝洛尔和α1-肾上腺素受体拮抗剂哌唑嗪可显着减弱高热。 TRPV1 KO 小鼠具有正常的基础体温，表明发育补偿。 d-安非他明（强效拟交感神经药）在 WT 小鼠中引起体温过高，而在 TRPV1 KO 小鼠中体温过高，表明 KO 小鼠的交感神经驱动力降低。这项研究提供了新的证据，表明 TRPV1 控制 SNS 上游的体温调节，为交感神经亢进性体温调节障碍提供了潜在的治疗靶点。—Alawi, K. M.、Aubdool, A. A.、Liang, L.、Wilde, E.、Vepa, A.、Psefteli, M.-P.、Brain, S. D.、Keeble, J. E. 交感神经系统由瞬态控制 受体电位香草酸1在体温调节中的作用。
Transient receptor potential vanilloid 1 (TRPV1) is involved in sensory nerve nociceptive signaling. Recently, it has been discovered that TRPV1 receptors also regulate basal body temperature in multiple species from mice to humans. In the present study, we investigated whether TRPV1 modulates basal sympathetic nervous system (SNS) activity. C57BL6/J wild-type (WT) mice and TRPV1 knockout (KO) mice were implanted with radiotelemetry probes for measurement of core body temperature. AMG9810 (50 mg/kg) or vehicle (2% DMSO/5% Tween 80/10 ml/kg saline) was injected intraperitoneally. Adrenoceptor antagonists or vehicle (5 ml/kg saline) was injected subcutaneously. In WT mice, the TRPV1 antagonist, AMG9810, caused significant hyperthermia, associated with increased noradrenaline concentrations in brown adipose tissue. The hyperthermia was significantly attenuated by the β-adrenoceptor antagonist propranolol, the mixed α-/β-adrenoceptor antagonist labetalol, and the α1-adrenoceptor antagonist prazosin. TRPV1 KO mice have a normal basal body temperature, indicative of developmental compensation. d-Amphetamine (potent sympathomimetic) caused hyperthermia in WT mice, which was reduced in TRPV1 KO mice, suggesting a decreased sympathetic drive in KOs. This study provides new evidence that TRPV1 controls thermoregulation upstream of the SNS, providing a potential therapeutic target for sympathetic hyperactivity thermoregulatory disorders.—Alawi, K. M., Aubdool, A. A., Liang, L., Wilde, E., Vepa, A., Psefteli, M.-P., Brain, S. D., Keeble, J. E. The sympathetic nervous system is controlled by transient receptor potential vanilloid 1 in the regulation of body temperature.