The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency
The 5T4 oncofoetal antigen is an early differentiation marker of mouse ES cells and its absence is a useful means to assess pluripotency
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DOI：
10.1242/jcs.00767
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发表时间：
2003-11-15
影响因子：
4
通讯作者：
Stern, PL
中科院分区：
文献类型：
--
作者：
Ward, CM;Barrow, K;Stern, PL
5 T4癌滋养层抗原是一种跨膜糖蛋白，由滋养层细胞和许多癌组织表达，但大多数正常成人组织不表达。人和小鼠5 T4 cDNA在细胞系中过表达的结果与其对粘附、形状和运动性的影响一致。我们表明，小鼠胚胎干细胞系是5 T4阴性，但有快速上调的蛋白质和转录后分化，包括衍生物的每个初级胚层，细胞表面的流式细胞仪，Western和RT-PCR分析证明。分化动力学和5 T4表达密切相关，早期事件将5 T4表达与运动性和形态学变化联系起来。将5 T4表达与其他ES细胞转录物（Oct 3/4;雷克斯-1）和抗原标记物（Forsmann，SSEA-1）进行比较，确立了5 T4作为ES细胞早期分化的非破坏性检测的有用标记物。例如，定义为SSEA-1阳性和5 T4阴性的“未分化”ES表型在嵌合体形成方面比SSEA-1阳性/5 T4阳性细胞的效率高7倍。因此，5 T4糖蛋白表达与ES细胞的早期分化事件相关，涉及运动性改变，并且它对于评估ES效力和研究发育和转移中的类似过程具有有用的实际结果。
5T4 oncotrophoblast antigen is a transmembrane glycoprotein expressed by trophoblast and, many carcinomas but not most normal adult tissues. Results from overexpression of human and mouse 5T4 cDNA in cell lines are consistent with it having an influence on adhesion, shape and motility. We show that murine embryonic stem cell lines are 5T4 negative but that there is rapid up regulation of protein and transcripts upon differentiation, including derivatives of each primary germ layer, as evidenced by cell surface FACS, western and RT-PCR analyses. The kinetics of differentiation and 5T4 expression are closely correlated, with early events linking 5T4 expression to changes in motility and morphology. Comparison of 5T4 expression with other ES cell transcript (Oct 3/4; Rex-1) and antigen markers (Forsmann, SSEA-1) establishes 5T4 as a useful marker for the non-destructive detection of early differentiation of ES cells. For example, 'undifferentiated' ES phenotype defined as SSEA-1 positive and 5T4 negative is seven times more efficient at chimera formation than SSEA-1-positive/5T4-positive cells. Thus, 5T4 glycoprotein expression is associated with early differentiative events of ES cells involving altered motility, and it has useful practical consequences for assessing ES potency and studying similar processes in development and metastasis.