Endonuclear symbiotic bacteria Holospora species distinguish the host nuclear envelopes
Endonuclear symbiotic bacteria Holospora species distinguish the host nuclear envelopes
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核内共生细菌 Holospora 物种区分宿主核膜
DOI：
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
M. Kawai
中科院分区：
文献类型：
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作者：
M. Fujishima;M. Kawai
革兰氏阴性细菌 Holospora 物种可以区分纤毛虫草履虫物种的两种细胞核：大核和微核。为了了解全孢菌属物种如何区分其目标核膜的机制，开发了针对大核特异性 H. obtusa 和微核特异性 H. undulata 外膜的单克隆抗体。间接免疫荧光显微镜显示，当提取物与分离的大核和微核混合时，从每个全孢菌属物种的 SDS-PAGE 凝胶中提取的外膜物质与其目标核膜结合。免疫印迹显示，H. obtusa 中抗原的相对分子质量为 16 kDa，H. undulata 中抗原的相对分子质量为 13 kDa。这些抗原具有蛋白酶 K 抗性，并且未用考马斯亮蓝-R250 或普通银染色染色。然而，这些抗原用细菌脂多糖银染色。我们的结果表明，全孢菌种的细胞核特异性感染是由全孢菌种感染形式外膜脂多糖与宿主细胞核被膜上未知受体物质之间的亲和力控制的。
s The Gram-negative bacterium Holospora species can distinguish two kinds of nuclei, a macro- and a micronucleus, of the ciliate Paramecium species. To know mechanism how Holospora species can distinguish their target nuclear envelopes, monoclonal an- tibodies specific for the outer membranes of the macronucleus-specific H. obtusa and the micronucleus-specific H. undulata were developed. Indirect immunofluorescence microscopy showed that the outer membrane substances extracted from SDS-PAGE gels of each Holospora species bound to their target nuclear envelopes, when the ex- tracts were mixed with isolated macro- and micronuclei. Immunoblots showed that relative molecular masses of the antigens were 16 kDa in H. obtusa and 13 kDa in H. undulata. These antigens were proteinase K resistant and unstained with Coomassie brilliant blue-R250 or with ordinary silver staining. However, these antigens were stained with silver for bacterial lipopolysaccharide. Our results showed that nu- cleus-specific infection of Holospora species was controlled by an affinity between lipopolysaccharides of the outer membranes of the infectious forms of Holospora spe- cies and the unknown receptor substances on the nuclear envelopes of the host cell.