Elevation of adenylate cyclase activity during leukemic cell differentiation.
Elevation of adenylate cyclase activity during leukemic cell differentiation.
复制标题
白血病细胞分化过程中腺苷酸环化酶活性升高。
DOI：
10.1016/0014-4827(87)90021-8
复制
发表时间：
1987
影响因子：
3.7
通讯作者：
Durham,J
中科院分区：
文献类型：
--
作者：
Fontana,J;Miksis,G;Durham,J
将各种人髓性白血病细胞系（HL 60和RDFD）暴露于各种化合物导致细胞的显著分化。这种分化与基础和NaF刺激的腺苷酸环化酶（AC）活性的显着增加有关。AC活性的增加与所使用的分化诱导剂（视黄酸（RA）、二甲基甲酰胺（DMF）、次黄嘌呤（HPX）或放线菌素D（actD））无关，并且与该过程相关，因为在暴露于各种诱导剂时不分化的HL 60细胞（HL 60-Blast）的变体不显示AC活性的这种增加。此外，分化过程与细胞内cAMP在加入诱导剂后数小时内迅速增加，随后逐渐减少有关。
Exposure of the various human myeloid leukemic cell lines (HL60 and RDFD) to various compounds results in marked differentiation of the cells. This differentiation is associated with a marked increase in both basal and NaF-stimulated adenylate cyclase (AC) activity. The increase in AC activity occurs regardless of the differentiation inducer one has utilized (retinoic acid (RA), dimethyl formamide (DMF), hypoxanthine (HPX) or actinomycin D (actD) and is correlated with this process, as a variant of the HL60 cell (HL60-Blast) that does not differentiate upon exposure to the various inducers does not demonstrate this increase in AC activity. In addition, the differentiation process is associated with a rapid increase in intracellular cAMP within hours of adding the inducer, followed by a gradual decrease.